Davids Biotechnologie was founded 1996 in Regensburg, a world cultural heritage city in Germany. We offer all kind of custom antibody services. Polyclonal antibodies in rabbits, chicken, mice, rats or guinea pigs. Monoclonal Antibody development in mice or rats. Any kind of customer provided or Davids made antigens are possible. We developed animal and ecologically friendly protocols to produce the antibodies. In addition all antibodies are made directly from the manufacturer. This leads to efficient methods and low prices. Furthermore Davids developed methods to produce antibodies within 28 days (SuperFast) or with a very low amount of antigen (LowDose).
Our purification methods are very well established and we offer Affinity, ProteinA, Depletion, Size-Exclusion, Ion-Exchange, IgM or any other purification that fits to your antibody project.
We offer different kind of antigen production services. Peptide antibodies, protein expression, SDS-extractions and more to generate your antigens for the antibody production.
Additionally we modify your antibodies like fragmentation, enzyme or dye conjugations. In our Shop you can buy antibodies, proteins, kits or buffers.
Antibody Information - Issue IV
Phosphorylation of proteins can play an important role in the recognition of protein activity or in their stability. Phospho-Antibodies can thus recognize the status of phosphorylation sites. Our all-complete phospho-specific antibody service for rabbit, chicken, mice, rats or guinea pigs include every step needed for your phosphospecific antibodies:
Synthesis and conjugation of your phospho-peptide
Synthesis of the non-phosphopeptide for depletion
Immunization of the animal of choice
Affinity Purification with the phospho-peptide
Depletion with the non-phosphopeptide to remove non-specific antibodies
You receive Phosphospecific Antibodies, Aliquot of the non-phosphopeptide, aliquot of the phosphopeptide, preimmune and a protocol
This time we focus peptide antibodies: We are able to produce antibodies against proteins you only know the sequence from. There is no need for protein production or purification. We help you to find peptides that represent your protein (see info box). These peptides have to be antigenic, soluble and must have good epitopes. For a higher chance to use the best peptide you can immunize one animal with two different peptides. This doubles your chance to use the best peptide for your applications.
The synthesized peptides are conjugated to a carrier molecule like KLH to improve the antigen presentation of the small peptides. The synthesis, conjugation and/or the immunizations can be performed by Davids.
We can produce peptide antibodies from rabbits, chicken, mice or other animals. Just send us your protein sequence (amino-acid one letter code prefered) and we analyze the sequence for free and handle your data confidentially. An order is only placed when you confirm one or more peptides. For further information go to our peptide section: Peptide Antibodies
Affinity Purification is a chromatography, which is used to seperate molecules in a solution. The molecule of interest (i. e. antibodies) make a specific interaction to the affinity matrix (i. e. antigen covalently bound to the affinity matrix). All non-specific molecules (i. e. antibodies that do not bind the antigen) cannot bind the affinity matrix and are washed. The molecule of interest is eluted afterwards. Besides the purification of antibodies affinity purification can be used for enzyme-substrate or receptor-ligand interaction.
Affinity purified antibodies have large advantages in many applications. Usually you can use higher concentrated antibodies in your applications like IHC, WesternBlot or DotBlots with reduced background.
The first step of the affinity purification is the creation of the affinity matrix. For each antibody a specific matrix is produced. The matrix material is incubated with the antigen under special conditions. Under these conditions covalent bindings are formed between the matrix and the antigen. In the next step the matrix is used to purify the antisera or the PrepI from chicken egg yolk. The specific antibodies (red in the figure) bind the antigen (orange in the figure) covalently bound to the matrix. The unspecific antibodies (blue in the figure) are washed and the specific antibodies can be eluted afterwards.
For more information on our purifications you can go to our Purification section.
We are able to use your protein specific SDS-PAGE gel bands as antigen. The advantage is that you save time with complicated purification steps. In addition you are able to get rid of even small protein contaminations.
The gel extraction is simple. Prepare a solution with at least 0.5 mg protein for the immunization of one rabbit for example and additional 0.5 mg protein if you want to order an affinity purification. Just run multiple bands with your protein solution, dye the gel with coomassie and cut all bands that belongs to your protein from the SDS-PAGE gel. Send the gel pieces in a 2-ml or 15-ml tube at room temperature without the addition of any solvents to Davids Biotechnologie GmbH, Röntgenstrasse 3, 93055 Regensburg, Germany.
The final antibodies are usually very good for applications like western blot and all other fields where you need to detect the denaturated protein. Even though for some applications where you need to detect the protein in its native form the immunization of the native protein has advantages.