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Monoclonal Antibody Development
Monoclonal Antibody Development
Email us for your individual offer
Monoclonal Antibody Development Order Form
Monoclonal Antibody Production
Monoclonal Antibody Purification

Monoclonal Antibodies from mouse and rat

We develop your custom monoclonal antibodies from mice or rats with any kind of antigen. To receive the best results we alter the concentration of the antigen for the immunizations. This ensures optimal immune response and we continue with the best mouse. In addition you can start or stop with each step. This enables you to send us spleen cells and we just perform the fusion and screening. Or we perform the immunization and you continue with the fusion and screening in your own lab.
The development of monoclonal antibodies is very specific for each customer. We can adapt our protocols for your special needs. This includes immunization protocols, screening conditions or alternative screening substrates.
Antigen Preparation

Antigen Requirements

Immunization

Immunization Schedules

Fusion

Fusion of Spleen Cells

Screening

Screening & Subcloning

Order

Order Process



Antigen Requirements for our Monoclonal Services

We successfully used any kind of antigen for the development of monoclonal antibodies. Recombinant proteins and Peptides that represents epitopes of the protein are the most common antigens for monoclonal antibodies. Please have a look at our table to see the requirements for the antigens. It is possible that you first send us your antigen for the immunizations and then send us the antigen needed for the screening. If your antigen is expensive or hard to produce please ask us for efficient immunization and screening to improve the amount of antigen that is needed.

Antigens for the Development of Monoclonal Antibodies
Antigen Amount Instructions Shipping
Protein1 - 2 mg
0.3 - 2 mg/ml
We use your protein for the immunization and Screening-20°C (ice packs) to -80°C (dry-ice) or lyophilized
Protein Expression-We produce your antigenYou can ship your expression plasmid at -20°C
Peptides3 - 5 mg
0.3 - 2 mg/ml
We can synthesize and conjugate your peptides or use your onesLyophilized or with ice packs
Cells106 cfu / immunizationUse our cell immunization kit to prepare for immunizationsPlease contact us when you want to send cells
Small Molecules1 - 5 mg
0.3 - 2 mg/ml
We prepare your small molecules for the immunizationPlease contact us when you want to send small molecules
For Affinity Purification we need additional >0.5 mg protein, 3 mg peptide or 108 cfu

STEP 1: Immunization

Titer Development after Immunization We immunize 4 BALB/c mice or 4 black hooded rats with different antigen concentrations to have a broad spectrum of immune responses. We perform ELISA titer determinations to determine the animal with the best response. You are able to receive an aliquot of the test bleed to determine the best titer in your lab as well. With your confirmation we continue with the immunizations or start the fusion and screening.
Monoclonal Antibody Immunization Schedule
STEP Day Description
STEP1: Immunizations
PreimmuneDay 0
1st ImmunizationDay 1
2nd ImmunizationDay 14
3rd ImmunizationDay 21
Test Bleed & ELISA titer determinationDay 28
4th ImmunizationDay 39
5th ImmunizationDay 60Optional
6th ImmunizationDay 68Optional

STEP 2: Spleen Cell Isolation & STEP 3: Fusion

1 day

Monoclonal Antibody Fusion The animal with the best immune response receives a final immunization to boost the antibody producing cells. The spleen cells are isolated for direct fusion with immortal myeloma cell lines. A part of the spleen cells is cryoconserved as a backup. The 3 remaining animals are kept as a backup until the fusion finished.
Alternatively for direct fusion or RNA isolation in our lab you are able to receive the isolated spleen cells for your own use in your lab.

STEP 4: Screening for positive clones

Approximately 2 - 4 months

Monoclonal Antibody Screening We grow all fusion cells and screen for positive clones in cell culture plates (alternative screening methods are available). Positive clones are grown in flasks and screened for stability and antigen specificity. We are able to screen against multiple antigens. The best clones are isolated and cryoconserved. You receive 5-ml supernatant from the best 5 - 10 clones for your own applications and to determine which clone is the best for the subcloning. In addition you receive our screening protocol to be up to date and to receive the screening raw data for your protocols. You are able to receive the raw clones before subcloning and perform the subcloning in your lab or to work with the raw clones.

STEP 5: Subcloning

Approximately 1 - 2 months

Monoclonal Antibody Subcloning We perform subcloning with the limited-dilution method, which ensures that the clone is truly monoclonal and improves the long-term stability. After subcloning you receive all clones frozen or in cell culture for your own lab. The rights of the clones belong to the customer after payment.
We store the clones for 8 months as a backup. Further storage is possible. This enables you to order an antibody production and purification in our lab from your antibody producing clones.

Production & Purification of your Antibodies

Monoclonal Antibody Production We offer the production and purification of the antibodies from your antibody producing clone. We established different purification steps to purify IgG and IgM antibodies. For further information go to our Monoclonal Antibody Production page.

Additional Services

We offer multiple additional services for your special needs. For toxic antigens we offer in vitro immunizations or the inclusion of the antigen in liposomes. In addition you are able to receive isolated cells from bone marrow, spleen, lymph nodes and PBMC.
The ELISA raw data are inclusive in our monoclonal immunization service M001.

Additional Services for Monoclonal Antibodies
Cat.No. Description Amount Price
A057Additional Small BleedAnimal DependingPlease ask
A705Additional ELISA Raw Data incl. graphFrom 1 Bleed17 EUR
N101Spleen Cell Isolation1 spleen172 EUR
N201PBMC Lymphocyte Isolation1 antiserum172 EUR
N301Bone Marrow Cell Isolation1 animal215 EUR
N401Lymph Node Cell Isolation1 animal172 EUR
M006Subclass Determination IgM/IgG1 clone21 EUR
M008Storage of cell line for one year1 clone, 3 vials69 EUR
M302Mycoplasm Test (PCR)1 cell culture103 EUR

Order Process

You can get your offer for your monoclonal antibodies here: info@davids-bio.com. We keep the order process very simple. You can send us your antigen to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The immunizations usually start the same day the antigen arrives. To fasten up the immunization process and help us to connect your antigen with your order ensure you provide the following information:

  1. Your Antigen
  2. Concentration & Buffer Conditions of your Antigen
  3. Our Offer, our order form or your official PO
  4. VAT number (only EU Customers, not German Customers)

If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies

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Davids Biotechnologie GmbHemail:info@davids-bio.com
Roentgenstrasse 3phone:+49-941-948228
93055 Regensburgfax:+49-941-9468119
Germany