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Monoclonal Antibody Production
Monoclonal Antibody Production
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Monoclonal Antibody Development

Production & Purification

Davids Biotechnologie offers the production of antibodies from your antibody producing hybridoma clones since 1996. In addition we offer transient transfection to produce your antibody or protein in CHO or HEK cells. You can use cells, hybridomas and clones from your lab or use clones developed by Davids.
Production

Antibody Production

Purification

Antibody Purification

Transient Transfection

Transient Transfection

Services

Additional Services

Order

Order Process



Antibody Production from Clones

We produce and purify antibodies from your antibody producing hybridoma. You can either send your hybridoma clone to Davids for the production or use one that was developed and stored by Davids against your antigen (Monoclonal Antibody Development). For the production we use exclusively spinner & roller flasks in inhouse developed bio reactors. The results are pure antibody fractions with low endotoxin. We offer a wide range of production from 10 mg to 10000 mg. Just ask for your special offer for large scale productions. In the table 1 you find some examples for the antibody production & purification service. In addition we offer the production in serum free medium and the production and purification of IgM or antibodies, that do not bind to ProteinA.

Monoclonal Antibody Production
Production (5 - 10 mg) Production (30 mg) Production (100 mg)
Cat.No.M301-010M301-030M301-100
We needYour clone frozen (-80°C)Your clone frozen (-80°C)Your clone frozen (-80°C)
Schedule
Growing2 weeks2 weeks2 weeks
Production1 - 3 weeks2 - 3 weeks2 - 4 weeks
Purification1 weeks1 weeks1 weeks
Product
PurificationProteinA includedProteinA includedProteinA included
Targeted Amount5 - 10 mg30 mg100 mg
Quality ControlDetermination of Protein ConcentrationDetermination of Protein ConcentrationDetermination of Protein Concentration
Mycoplasm Test+++
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We are able to make you special prices for the production of larger or smaller scales. Please keep in mind that the schedule and the yield is dependent on the antibody producing clone. In addition ProteinA does not work with every clone. For these clones please have a look at our antibody purification services.

Purification of Supernatants

The purification (ProteinA) of your supernatants from your antibody producing clones is included. In some cases you need additional polishing steps to get rid of polymers or other disturbing substances. For this we offer our polishing purification that can be done after the ProteinA.
For IgM or antibodies that do not bind to ProteinA we offer our ProteinA Independent Purification. Here we are able to purify IgM, small antibody pieces and more.

Monoclonal Antibody Purification
ProteinA Independent Purification Polishing
Cat.No.M501M502M503
Schedule
Schedule1 weeks1 - 2 weeks1 - 2 weeks
Product
Available forIgGIgG
IgM
Fragmented Antibodies
IgG
IgM
Fragmented Antibodies
MethodProteinAIon-Exchange-Chromatography (IEX)
Size-Exclusion-Chromatography (SEC)
Ion-Exchange-Chromatography (IEX)
Size-Exclusion-Chromatography (SEC)
Amount
We are able to purify small and large amounts
Quality ControlSDS-PAGE & Protein ConcentrationSDS-PAGE & Protein ConcentrationSDS-PAGE & Protein Concentration
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Transient Transfection

The production of proteins and antibodies by transient transfection in suspension cultures of HEK or CHO cell cultures offers the advantage of correctly posttranslational modified proteins. Protein yields are 1 to 200 mg from 1 liter medium. You can send your transient transfection plasmid to us. We produce your protein. Alternatively you can send us your plasmid in small amounts (50 ng) and we produce your plasmid for the transient transfection.

Transient Transfection
Plasmid Amplification Transient Transfection in CHO Transient Transfection in HEK
Cat.No.E500E501E502
We need50 ng of your transient transfection plasmid1 - 5 mg Plasmid1 - 5 mg Plasmid
Schedule5 days4 - 8 weeks4 - 8 weeks
Purification Production with reduced EndotoxinProteinA
Other Purifications Available
ProteinA
Other Purifications Available
Quality ControlAgarose GelSDS-PAGE & Protein ConcentrationSDS-PAGE & Protein Concentration
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Additional Services

Additional Services for Monoclonal Antibody Production
Cat.No. Description Amount Catalog Price
M006Subclass Determination: IgG/IgM1 clone21 EUR
M008Storage of one cryoconserved clone: 3 vials, 1 year1 clone, 1 year69 EUR
-Sterile Filtration1 fraction
B001ELISA Development: Antigen1 clone
B002ELISA Development: Primary Antibody1 clone
-WesternBlot Analysis1 clone
-Immuno-Histo-Chemistry Analysis1 clone
-Up-Concentration of IgG1 fraction

Order Process & Clone Shipping

We keep the order process very simple. You can send us your clone or supernatant to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

We start with the production and purification as soon as your material arrives. Please add the following information to help us connect your order with the material:

  1. Type of antibody
  2. If known type of wanted purification
  3. Our Offer, our order form or your official PO
  4. VAT number (only EU Customers, not German Customers)

You have the choice to send us your clone or use one of your clones developed or stored at Davids. You can ship your clone according to the table:

Antibody Producing Clone Shipping
Type
Frozen cellsFreeze the cells with 5 - 10% DMSO or 5% glycerin. You may add 10 - 20% serum.
Cell culturesCentrifuge the cells in log-phase (max. 300 x g), discard the supernatant and resuspend the pellet in fresh medium. Close the lid and send it in the vial together with a non-frozen ice pack.

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Davids Biotechnologie GmbHemail:info@davids-bio.com
Roentgenstrasse 3phone:+49-941-948228
93055 Regensburgfax:+49-941-9468119
Germany