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Antibody Purifications
Antibody Purification
Purification Methods Theory
Production and Purification of Monoclonal Antibodies
Production of Polyclonal Antibodies

Purification

Davids Offers the purification of antibodies and proteins from antisera, egg yolk and supernatants. We are able to purify material from your lab or antibodies that were produced at Davids.
Antisera Purification

Antisera Purifications

Chicken Antibody Purification

Egg Yolk Purifications

Supernatant Purification

Supernatant Purification

Order Process

Order Process



Purification of Antisera

Purification of antisera is done for better results in your applications. Applications like WesternBlot, IHC, DotBlot or ELISA profit for purifications of the antisera. In addition you receive a pure antibody fraction depending which kind of purification you choose. The table shows our most ordered products, though we purify larger amounts of antisera, supernatants and egg yolks as well. Just ask for your special offer. For our antibody production services including the immunizations go to our Rabbit or Small Rodent (Mice, Rats, Guinea pigs) section.

Antisera Purification
Affinity Purification ProteinA Cell Culture Purity Depletion
Cat.NoR007 (10 - 50 ml)
R007-2 (50 - 100 ml)
R008 (10 - 50 ml)R003 (10 - 50 ml)R101 (anti-tag)
R102 (anti-E. coli)
Duration5 - 10 days5 days5 - 10 days5 days
Product Information
ColumnAntigen bound to resinProteinA bound to resinAntigen bound to resintag/E. coli bound to resin
Additional InformationPlease send your antigen (0.5 - 1 mg) for the creation of the affinity matrixPlease send your antigen (0.5 - 1 mg) for the creation of the affinity matrix
ProductSpecific AntibodiesWhole IgG FractionSpecific Antibodies
Low Endotoxin
Sterile
Fraction without unwanted Antibodies
Applications
Optimal forWesternBlot
ImmunoHistoChemistry (IHC)
Fluorescent Activated Cell Sorting (FACS)
ELISA
ImmunoHistoChemistry (IHC)
in vivo Applications
Fluorescent Activated Cell Sorting (FACS)
Depends on Depleted Fraction
PricePrice ListPrice ListPrice ListPrice List
Get your offer with special prices now

Purification of Egg Yolk

We purify your egg yolk antibodies. Preparation I removes most lipids and lipoproteins. Preparation II removes remaining lipids and lipoproteins and make a polishing step. Preparation III performs an affinity purification at a low endotoxin level and sterile filtration for direct use in cell culture. In addition we offer affinity purifications for your egg yolk antibodies. For full chicken egg yolk antibody services including the immunizations go to our Chicken Egg Yolk Antibody Section.

Purification Options for Chicken Egg Yolk Antibodies
PrepI PrepII PrepIII Affinity Purification
Cat.NoR001R003R006R017
Duration5 days5 days5 - 10 days1 - 5 days
Product Information
ProcedurePreparationChromatographyAntigen bound to resinAntigen bound to resin
Comparable withRaw SerumProteinAPrepIIIAffinity Purification
ProductRaw Antibodies in bufferHigh purity, raw antibodiesSpecific Antibodies
Low Endotoxin
Sterile
Specific Antibodies
Purity70 - 90%90 - 98%
Amount (approx.)8 eggs corresponds to 100 ml Serum8 eggs corresponds to 100 ml Serum0.1 - 10 mg (8 eggs)0.5 - 10 mg (8 eggs)
Applications
Optimal forELISAImmuno-Histo-Chemistry (IHC)
in vivo Applications
Fluorescent Activated Cell Sorting (FACS)
WesternBlot
Immuno-Histo-Chemistry (IHC)
Fluorescent Activated Cell Sorting (FACS)
ELISA
PricePrice ListPrice ListPrice ListPrice List
Get your offer with special prices now

Purification of Supernatants from Monoclonal Antibodies

We offer the purification of your monoclonal antibody supernatants or the production & purification of your monoclonal antibody producing clone. Please have a look at our Monoclonal Antibody Production & Purification section. Alternatively you can leave the antibody development to us with our Monoclonal Antibody Development Service.

Order Process

We keep the order process very simple. You can send us your material to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The purification usually starts as soon as your material arrives. To fasten up the process and help us to connect your material with your order ensure you provide the following information:

  1. Your Antigen for affinity purifications only
  2. Your Antibody containing solution
  3. Your desired Purification
  4. Our Offer, our order form or your official PO
  5. VAT number (only EU Customers, not German Customers)

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Davids Biotechnologie GmbHemail:info@davids-bio.com
Roentgenstrasse 3phone:+49-941-948228
93055 Regensburgfax:+49-941-9468119
Germany