Protein Expression for your Custom Antibody Production
Recombinant expression of your protein in E. coli is used to receive enough protein for the production of antibodies. As an alternative to protein expression in E. coli you can use peptides that represent your protein. Just send us your protein sequence and we suggest you peptides for the antibody production. For more information go to our Peptide Page.
Cat.No.E111: DNA Synthesis Cat.No.E112: Subcloning
We synthesize your DNA and optimize the codon for the expression in E. coli. We insert restriction sites to subclone the synthesized DNA in a vector. The DNA is subcloned in an expression vector. Here we usually use an IPTG induceable plasmid with ampicillin or kanamycin resistance and a tag for easy purification.
Protein Expression Evaluation
Cat.No.E113: Expression Evaluation
To express your protein in the best way possible we perform an expression evaluation. The plasmid is transformed in different E. coli strains (two strains are included). The inductor concentration, the expression time and the expression temperature is varied.
This is only needed, when no expression protocol is available. When you provide us with an the expression plasmid and an expression protocol this step is not needed.
Protein Expression & Purification
Cat.No.E100: Protein Expression in E. coli
We perform the protein expression of your protein in E. coli after the protocol established in the Expression Evaluation or after a customer provided protocol. The expression is for the antibody production only. We can not guarantee that the final protein/enzyme stays active during the purification process.
You can get your offer for protein expression here: firstname.lastname@example.org. Every expression project is different and to give you the best service I recommend to write us your special needs.
To fasten up the process and help us to give you the best service possible ensure you provide the following information:
If available: Expression Plasmid, Expression Protocol, Plasmid information (Sequence, Origin, Antibiotic Resistance, Inductor/Promotor, tag)
Sequence from your gene
Known Information of your antigen: Solubility, Toxicity
Our Offer or your official PO
VAT number (only EU Customers, not German Customers)
As an alternative to protein expression you can use peptides that represent your protein. If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies