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Protein as antigen
Protein As Antigen
Antigen Overview
Peptide Synthesis Service
Expression Service

Protein as Antigen

Proteins in its native or denaturated forms are usually the antigen of choice when the protein is available. The immunogenicity is good as multiple epitopes are available on the protein. In addition the accessibility of the final antibodies to your antigen in your applications is very high when you use the same antigen. The immunization can be done with any purity. Though the chances are high that antibodies are generated against the impurities. Consider to run an SDS-gel and send us the antigen specific gel bands as antigen when your purity is low.
For tagged proteins we can perform a depletion when the anti-tag antibodies disturb in your assays.

Whole Protein

Whole
Protein

SDS Protein

Proteins from SDS-gels

Peptide Conjugation

Alternatives to Proteins

Additional Information

Additional Information

Order

Order Process


Whole Protein

You can send your whole protein or a part of your protein to Davids Biotechnologie GmbH, Roentgenstrasse 3, 93055 Regensburg, Germany. We start the immunizations as soon as your antigen arrives. Please have a look at our buffer requirements: Buffer Requirements before you send the antigen. For tagged antigens please have a look at our additional information.
Proteins can usually be send with ice packs, lyophilized or on dry ice.


Rabbit Chicken Mouse Rat Guinea Pig
Rabbit Antigen RecommendationsChicken Antigen RecommendationsMouse Antigen RecommendationsRat Antigen RecommendationsGuinea Pig Antigen Recommendations
For Immunizations (1 animal)0.2 - 0.6 mg0.2 - 0.6 mg20 - 250 µg20 - 250 µg0.15 - 0.5 mg
For Affinity Matrix0.5 - 2 mg0.5 - 2 mg0.5 - 2 mg0.5 - 2 mg0.5 - 2 mg
Concentration0.3 - 2 mg/ml0.3 - 2 mg/ml0.3 - 2 mg/ml0.3 - 2 mg/ml0.3 - 2 mg/ml
Buffer
Any physiological non-toxic buffer like 1 x PBS or 0.9% NaCl. For more information: antigen recommendations

Proteins from SDS-gel

Cat.No.A703

Gel pieces from SDS-gels are easy to use and have some advantages. You save time with complicated purification steps. In addition you are able to get rid of even small protein contaminations.
The gel extraction is simple. For example: Prepare a solution with at least 0.5 mg protein for the immunization of one rabbit and additional 0.5 mg protein if you want to order an affinity purification. Just run multiple bands with your protein solution, dye the gel with coomassie and cut all bands that belongs to your protein from the SDS-PAGE gel. Send the gel pieces in a 2-ml or 15-ml tube at room temperature without the addition of any solvents to Davids Biotechnologie GmbH, Roentgenstrasse 3, 93055 Regensburg, Germany.
The final antibodies are usually very good for applications like western blot and all other fields where you need to detect the denaturated protein. Even though for some applications where you need to detect the protein in its native form the immunization of the native protein has advantages.

Affinity Purification Scheme

Alternatives when the protein is not available

When your protein is not available you can use our peptide synthesis service. Here we use peptides that represent your antigen. When you have the expression plasmid available we can express your protein for the antibody production. Just use our expression service. If you have other antigens just have a look at our antigen overview or write us an email with personal suggestions: info@davids-bio.com.

Additional Information

tagged Proteins

Purified proteins with tags as antigen leads to an antibody fraction with antibodies recognizing the protein and the tag. When these anti-tag antibodies disturb in your assays we have multiple ways to remove these antibodies. Our depletion service Cat.No.R101 removes the anti-tag antibodies with our tag-depletion chromatography.
If you order an affinity purification we can remove the anti-tag antibodies when we use a protein without a tag or with another tag for the affinity purification. I. e. we use a HIS-tagged protein for the immunization and a non-tagged or STREP-tagged protein for the affinity purification.

Small Proteins

For proteins smaller than 10 kDa we recommend to perform a conjugation with your protein. The conjugation of the protein activates the immune system of the animals in its best way. For more information please go to our conjugation services.

Purity needed for antibody production

We produce antibodies from antigens with any purity. Please keep in mind that antibodies against all impurities are made as well. To receive specific antibodies even with less pure proteins we recommend our SDS-gel extraction service or a subsequent affinity purification with pure protein.

Order Process

We keep the order process very simple. You can send us your antigen to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The immunizations usually start the same day the antigen arrives. To fasten up the immunization process and help us to connect your antigen with your order ensure you provide the following information:

  1. Your Antigen
  2. Concentration & Buffer Conditions of your Antigen
  3. Our Offer, our order form or your official PO
  4. VAT number (only EU Customers, not German Customers)

If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies

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Davids Biotechnologie GmbHemail:info@davids-bio.com
Roentgenstrasse 3phone:+49-941-948228
93055 Regensburgfax:+49-941-9468119
Germany