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Custom Antibodies
Polyclonal Antibodies
We offer polyclonal antibodies for more than 20 years. We developed cost efficient and animal friendly methods to produce high quality polyclonal antibodies in different species. As a customer from Davids Biotechnologie you are able to choose between superior services for your individual needs.
For additional questions or your individual offer please write us an email: info@davids-bio.com

Polyclonal Antibodies

Polyclonal antibodies are the antibodies of choice when you need inexpensive and fast produced high affinity antibodies. Your polyclonal antibodies recognize multiple epitopes of your antigen, which can lead to a better detection in some applications. Multiple antibodies are able to bind your protein at different epitopes which leads to a signal amplification. This usually leads to better results in immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP).

Antigen Preparation

Antigen Preparation

Immunization

Immunization
 

Antibody Purification

Antibody Purification

Antibody Preparation

Additional Services



Antigen

For the antibody production nearly any kind of antigen can be used. Usually proteins, peptides, SDS-Gel pieces, DNA, cells or small molecules are used for polyclonal or monoclonal antibodies.
When you have purified protein available, the native protein can be used as antigen. When the purification of the protein is difficult or when the purified protein shows multiple bands in a SDS-gel you can use our SDS-Gel Extraction service (Cat.No.A703). Here you cut your protein specific gel band from your gel and we extract the protein from it.
For proteins you only know the sequence from you can use our Peptide Antibody Service (P101). This service includes a peptide prediction to see, which peptides represent your protein in the best way. Our scientist with the help of our analytical program predict the antigenicity, solubility and epitopes of the peptides. We synthesize and conjugate the peptides to a carrier to improve the antigenicity.

If you have any questions regarding your antigens you can just send us an email: info@davids-bio.com

Suitable antigens for Antibody Production
Antigen Cat.No. Description We need
ProteinProvided by CustomerWe use your protein for the immunizationYour antigen
Protein ExpressionE100Protein Expression Service for antibody productionYour expression plasmid
Protein (SDS-Gel)A703Protein Extraction from SDS-Gel piecesYour protein in SDS-gel pieces
PeptidesP101Peptide Prediction, Synthesis & ConjugationYour protein amino acid sequence
CellsK105Preparation of cells for immunizationYour inactivated cells
DNAA702Preparation of DNA for immunizationYour DNA
Small MoleculesP105Conjugation of the small molecules if neededYour antigen
If you need further information or if you have another antigen that does not fit in the table please contact us.

Immunization

We start with the immunizations as soon as your antigen arrives or as soon as we finished the synthesis or preparation of your antigen. We offer the immunization of different species for your special needs. Most common are “New Zealand White Rabbits” for the production of your polyclonal antibodies. In addition we offer the immunization of chicken, mice, rats or guinea pigs. We are able to immunize one or multiple animals, depending how many antibodies are needed. Please ask for your special offer for a large number of animals.
We have different protocols for the needs of our customers. Our standard 63-day protocol fits to most applications. For customers who need their antibodies faster we offer our SuperFast procedure, which takes 28-days. Here we use a superior adjuvant and a proprietary immunization schedule.

Antibody Immunization Schedules
Animal Product Purification Advantages
Rabbit40 - 90 ml antiserumProteinA, Affinity Purification
ChickenEgg Yolk from 8 eggs / 14 daysPrepI, PrepII, Affinity PurificationLarge Amounts of Antibodies over time
Mouse0.2 - 1 ml antiserumProteinA, Affinity PurificationMonoclonal Antibody Production is possible
Rat2 - 10 ml antiserumProteinA, Affinity PurificationMonoclonal Antibody Production is possible
Guinea pig5 - 15 ml antiserumProteinA, Affinity PurificationOptimal choice for IHC
Phosphospecific AntibodiesPeptide, AntiserumAffinity Purification, DepletionRecognition of phosphorylation site

Purification

We recommend purifications when you need specific antibodies especially for applications like ImmunoHistoChemistry (IHC), WesternBlot (WB), ImmunoPrecipitation (IP), Chromatin ImmunopreciPitation (ChIP) or Fluorescent Activated Cell Sorting (FACS). Even for other applications like ELISA purified antibodies have advantages. We offer different purification methods for your special needs:

Antibody Purification Methods
Cat.No. Purification Available for Product Advantages
R007Affinity PurificationAntisera, Egg YolkSpecific Antibodies against your antigenHigher concentration of your antigen specific antibodies, less background
R008ProteinAAntiseraWhole IgG fractionSpecific and unspecific antibodies
R101DepletionAntiseraRemoves unwanted antibodiesAntisera without unwanted antibodies
A012PrepIEgg YolkRemoval of lipids, saltsComparable with raw antisera from mammals
R003PrepIIEgg YolkWhole IgY fractionComparable with ProteinA purification
R004PrepIIIAntisera, Egg YolkSpecific antibodies for cell culture useSterile & specific antibodies directly for your cell culture use

Additional Services

Davids offers a wide range of antibody processing like enzyme or dye conjugation, modification or fragmentation.

In addition we offer you to prepare cells from spleen, blood, lymph nodes or to prepare whole organs from immunized animals. We can ship the cells or organs to you or offer the preparation of RNA from the fresh isolated cells.


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Davids Biotechnologie GmbHemail:info@davids-bio.com
Roentgenstrasse 3phone:+49-941-948228
93055 Regensburgfax:+49-941-9468119
Germany