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Antibody Production in Germany, Europe

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Mouse, Rat and Guinea pig antibody production

The animals are housed in our own animal department in Germany (Europe). This guarantees that antibody manufacturing is both animal-friendly and cost-effective. Individual monitoring of each animal and titer progression on a regular basis assures high quality antibodies with optimal titers. For antibody production, BALB/c mice and black hooded rats are used as a standard. Other strains are available on demand. Guinea pig vaccination is provided to enhance our rodent expertise. Rats and guinea pigs have the benefit of producing a bigger volume of antiserum from a single animal.
Please visit our Monoclonal Development Page for additional information on monoclonal antibody projects.

Antigen Preparation

Antigen Requirements

Learn more about our rodent antigen requirements.

Immunization

Immunization Schedules

Take advantage of our well-established immunization methods.

Antibody Purification

Available Purifications

Additionals Services

Additional Services

Isolation of cells from spleen and bone marrow as well as DNA preparation

Order

Order Process


Antigen Requirements

To achieve the best results, it is advised that numerous mice, rats, or guinea pigs be immunized with various antigen concentrations. The table below shows the average antigen quantity required for vaccination of one animal. The quantity of antigen may change depending on the type of antigen used. Davids has already successfully produced antibodies using a tiny amount of the required antigen. Please email us at info@davids-bio.com if you have less antigen. Click on the selected antigen for more details.


Recommended antigen amount for the antibody production in small rodents
Antigen 1 Mouse 1 Rat 1 Guinea pig Description
ProteinProtein100 - 250 µg
0.3 - 2 mg/ml
100 - 250 µg
0.3 - 2 mg/ml
200 - 500 µg
0.3 - 2 mg/ml
Proteins supplied by customer
Expression in E. coliProtein Expression---Protein expression from your plasmid
SDS-gelProtein (SDS-Gel)150 - 250 µg
3 - 8 gel bands
150 - 300 µg
3 - 8 gel bands
200 - 600 µg
3 - 8 gel bands
Protein extraction from SDS-gel pieces. Cut the protein specific gel bands from the gel.
PeptidePeptides1 - 2 mg
0.3 - 2 mg/ml
1 - 2 mg
0.3 - 2 mg/ml
1 - 2 mg
0.3 - 2 mg/ml
Customer provided peptides or peptide synthesis and conjugation service by Davids.
CellCells5 x 107 - 1 x 108 cfu5 x 107 - 1 x 108 cfu5 x 107 - 1 x 108 cfuPreparation with Davids Cell Immunization KIT for optimal presentation of the surface to the immune system.
DNADNA1 - 2 mg
0.3 - 2 mg/ml
1 - 2 mg
0.3 - 2 mg/ml
1 - 2 mg
0.3 - 2 mg/ml
DNA preparation for the immunizations by Davids
Conjugation of a small moleculeSmall Molecules1 - 5 mg
0.3 - 2 mg/ml
1 - 5 mg
0.3 - 2 mg/ml
1 - 5 mg
0.3 - 2 mg/ml
Preparation of small molecules by Davids
For Affinity Purification we need additional 0.5 - 2 mg protein, 3 mg peptide or 108 cfu

Immunization Schedules for Small Rodents

Our well-established 63-day vaccination strategy delivers the best results in terms of titer development and antigen affinity for the majority of antigens. We can follow your specific immunization protocols or immunization strategies for your special requirements. There are options for extended immunization schedules, extra vaccinations, further test bleeds, and specific immunization techniques. For large projects including a significant number of mice, rats, or guinea pigs, please contact us at info@davids-bio.com for a special discount.


Recommended immunization protocols
Mouse Mouse Rat Rat Guineapig Guinea Pig
Cat.NoA402 (2 mice)
A404 (4 mice)
A206 (2 rats)A202 (2 guinea pigs)
Schedule63-day63-day63-day
Schedule ProlongationPossiblePossiblePossible
Immunizations555
Titer Determinationday 35day 35day 35
Serum Amount0.2 - 0.7 ml2 - 8 ml5 - 15 ml
Purification Options
PurificationPrecipitation (R001)
Affinity Purification (R007)
ProteinA (R008)
Precipitation (R001)
Affinity Purification (R007)
ProteinA (R008)
Precipitation (R001)
Affinity Purification (R007)
ProteinA (R008)
Antigen Requirements
Suitable AntigensProtein
Protein (SDS-Gel)
Peptide
DNA
Cells
Small Molecules
Protein
Protein (SDS-Gel)
Peptide
DNA
Cells
Small Molecules
Protein
Protein (SDS-Gel)
Peptide
DNA
Cells
Small Molecules
Antigen Needed100 - 250 µg Protein200 - 500 µg Protein200 - 500 µg Protein
Antigen BufferNon toxic bufferNon toxic bufferNon toxic buffer
PricePrice ListPrice ListPrice List
LetterGet your free quotation now
Rodent immunization schedules
Day 63 Day Antisera
1Preimmune serum
11. Immunization
142. Immunization
283. Immunization
35
  • Test serum
  • ELISA-Titer determination
  • Optional: Test serum for the customer
424. Immunization
565. Immunization
63Anti serum
Get your offer to extend animal housing and the number of immunizations.

Antisera Purification

We provide various purification services for mouse, rat, and guinea pig polyclonal antisera. To increase yield, we propose pooling antisera from individual mice before purification. Separate purifications from each antisera are also available.
There are affinity purifications for specific antibodies and ProteinA purifications to get a pure IgG fraction, as well as depletions to remove undesired antibodies that identify tags.


Purification options for polyclonal antibodies from mice, rats and guinea pigs
Affinity Purification ProteinA Cell Culture Purity Depletion
Cat.NoR007 (1 antiserum pool)
R007-2 (2 antisera)
R008 (1 antiserum pool)R003 (1 antiserum pool)R101 (anti-tag)
R102 (anti-E. coli)
Antigen Specific Antibodies:

Scheme of Antigen Specific Affinity Purification
Whole IgG Fraction:

Scheme of ProteinA Purification
Antigen Specific Antibodies:

Scheme of Affinity Purification
Anti-tag Antibody Removal:

Scheme of Antibody Depletion
Tag: Protein Tag Antigen Specific Antibody: Specific Antibody Unspecific Antibody: Unspecific Antibody Tag Antibody: Tag Antibody Antigen: Antigen
Duration5 days5 days5 - 10 days1 - 5 days
Product Information
ColumnAntigen bound to resinProteinA bound to resinAntigen bound to resintag/E. coli bound to resin
ProductSpecific AntibodiesWhole IgG FractionSpecific Antibodies
Low Endotoxin
Sterile
Fraction without unwanted Antibodies
Applications
Optimal forWesternBlot
Immuno-Histo-Chemistry (IHC)
Fluorescent Activated Cell Sorting (FACS)
ELISA
Immuno-Histo-Chemistry (IHC)
in vivo Applications
Fluorescent Activated Cell Sorting (FACS)
Depends on Depleted Fraction
Catalog PricePrice ListPrice ListPrice ListPrice List
Get your free offer now

Additional Services

We provide a variety of supplementary services to meet your specific requirements. There are further immunizations, test bleeds, and animal housings offered. We also provide cell preparation from the spleen, blood, bone marrow, or lymph nodes for the generation of monoclonal antibodies or RNA isolation.


Additional services for antibodies from rodents
Cat.No. Description Amount Catalog Price
A057Additional Small Bleed-Price List
A059Additional Immunization1 ImmunizationPrice List
A705ELISA Raw Data incl. graphFrom 1 BleedPrice List
A706Animal Housing1 day, 1 animalPrice List
N101Spleen Cell Isolation1 spleenPrice List
N201PBMC Lymphocyte Isolation1 antiserumPrice List
N301Bone Marrow Cell Isolation1 animalPrice List
N401Lymph Node Cell Isolation1 animalPrice List

Order Process

Davids keeps the ordering procedure as easy as possible. Please send your antigen to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

Immunizations usually begin on the same day as the antigen arrives. Please submit the following information to assist us correlate your antigen with your order and accelerate the process:

  1. Your Antigen
  2. Concentration & Buffer Conditions of your Antigen
  3. Our Offer, our order form or your official PO
  4. VAT number (only EU customers, not required for German customers)

Create your own quote with our Antibody Creator. When you prefer to order peptide antibodies, send us your protein sequence and we will recommend peptides that represent your protein. For further information, please visit: Peptide Antibodies