Davids Biotechnologie GmbH
News Contact DE | EN
Immunization Protocol
Polyclonal Antisera from small rodents
Small Rodent Antibody Order Form
Purification of Antisera
Monoclonal Antibodies
Go back to Polyclonal Antibody Overview

Polyclonal Antibody Production in Mice, Rats and Guinea pigs

All animals are housed in our department. This ensures animal friendly and cost efficient housings. We are able to have individual looks on each animal, the immunization schedules and your specific needs. We use BALB/c mice and black hooded rats for your antibody production. To complete our services for small rodents we offer guinea pigs as well. The advantage of rats and guinea pigs are the larger amount of antiserum from one animal.
For monoclonal projects please go to our Monoclonal Development Page.
Antigen Preparation

Antigen Requirements

Immunization

Immunization Schedules

Antibody Purification

Available Purifications

Additionals Services

Additional Services

Order

Order Process


Antigen Requirements

For optimal immunization success we offer to immunize multiple mice, rats or guinea pigs with different antigen concentrations to receive best results. The antigen amount needed for one animal are listed in the table below. Depending which kind of antigen you choose we need different amount of antigen. We already successfully generated antibodies with even less amount of the recommended antigen amount. If you have less antigen please contact us: info@davids-bio.com. For further information click on the desired antigen.

Antigens for Small Rodent Antibody Production
Antigen 1 Mouse 1 Rat 1 Guinea pig Description
Protein20 - 250 µg
0.3 - 2 mg/ml
20 - 250 µg
0.3 - 2 mg/ml
0.15 - 0.5 mg
0.3 - 2 mg/ml
We use your protein for the immunization
Protein Expression---We produce your antigen from your plasmid
Protein (SDS-Gel)25 - 250 µg
3 - 8 gel bands
0.15 - 0.3 mg
3 - 8 gel bands
0.15 - 0.6 mg
3 - 8 gel bands
Cut your specific protein band from your SDS-gel
Peptides1 - 2 mg
0.3 - 2 mg/ml
1 - 2 mg
0.3 - 2 mg/ml
1 - 2 mg
0.3 - 2 mg/ml
Use our synthesis and/or conjugation service
Cells106 cfu / immunization106 cfu / immunization106 cfu / immunizationPlease contact us when you want to use cells as antigen
DNA1 - 2 mg
0.3 - 2 mg/ml
1 - 2 mg
0.3 - 2 mg/ml
1 - 2 mg
0.3 - 2 mg/ml
We prepare your DNA for the immunizations
Small Molecules1 - 5 mg
0.3 - 2 mg/ml
1 - 5 mg
0.3 - 2 mg/ml
1 - 5 mg
0.3 - 2 mg/ml
We prepare your small molecules for the immunizations
For Affinity Purification we need additional >0.5 mg protein, 3 mg peptide or 108 cfu


Immunization Schedules for Small Rodents

Our well established 63-day immunization schedule offers the best results in titer development. For your special needs we are able to follow your immunization protocols or immunization techniques. Longer immunization times, additional immunizations, additional test bleeds or special immunization techniques are available. We can immunize large amounts of animals. For large projects with mouse, rats or guinea pigs please ask for your special offer: info@davids-bio.com.

Immunization Protocols
Mouse Rat Guinea Pig
Cat.NoA401 (1 mouse)
A402 (2 mice)
A404 (4 mice)
A205 (1 rat)
A206 (2 rats)
A201 (1 guinea pig)
A202 (2 guinea pigs)
Schedule63-day63-day63-day
Schedule ProlongationPossiblePossiblePossible
Immunizations555
Titer Determinationday 35day 35day 35
Serum Amount0.2 - 1 ml2 - 10 ml5 - 15 ml
Purification Options
PurificationPrecipitation (R001)
Affinity Purification (R007)
ProteinA (R008)
Precipitation (R001)
Affinity Purification (R007)
ProteinA (R008)
Precipitation (R001)
ProteinA (R008)
Antigen Requirements
Suitable AntigensProtein
Protein (SDS-Gel)
Peptide
DNA
Cells
Small Molecules
Protein
Protein (SDS-Gel)
Peptide
DNA
Cells
Small Molecules
Protein
Protein (SDS-Gel)
Peptide
DNA
Cells
Small Molecules
Antigen Needed20 - 250 µg Protein20 - 250 µg Protein0.15 - 0.5 mg Protein
Antigen BufferNon toxic bufferNon toxic bufferNon toxic buffer
PricePrice ListPrice ListPrice List
Get your offer with special prices now
Rodent Immunization Schedules
Day 63 Day Antisera
1Preimmune serum
11. Immunization
142. Immunization
283. Immunization
35
  • Test serum
  • ELISA-Titer determination
  • Optional: Test serum for the customer
424. Immunization
565. Immunization
63Anti serum
Get your offer to extend animal housing and the number of immunizations.

Antisera Purification

We offer different purification services for your polyclonal antisera from mouse, rat and guinea pig. We recommend to pool antisera from multiple mice before the purification to enhance the yield. Separate purifications from single antisera are available as well.
You have the choice of an affinity purification for specific antibodies and ProteinA purifications to receive a pure IgG fraction or depletions to get rid of unwanted antibodies that i. e. recognize tags.

Purification Options for Polyclonal Antibodies from Mice, Rats and Guinea Pigs
Affinity Purification ProteinA Cell Culture Purity Depletion
Cat.NoR007 (1 antiserum pool)
R007-2 (2 antisera)
R008 (1 antiserum pool)R003 (1 antiserum pool)R101 (anti-tag)
R102 (anti-E. coli)
Duration5 days5 days5 - 10 days1 - 5 days
Product Information
ColumnAntigen bound to resinProteinA bound to resinAntigen bound to resintag/E. coli bound to resin
ProductSpecific AntibodiesWhole IgG FractionSpecific Antibodies
Low Endotoxin
Sterile
Fraction without unwanted Antibodies
Applications
Optimal forWesternBlot
ImmunoHistoChemistry (IHC)
Fluorescent Activated Cell Sorting (FACS)
ELISA
ImmunoHistoChemistry (IHC)
in vivo Applications
Fluorescent Activated Cell Sorting (FACS)
Depends on Depleted Fraction
Catalog PricePrice ListPrice ListPrice ListPrice List
Get your offer with special prices now

Additional Services

We offer multiple additional services for your special needs. Additional Immunizations, Test bleeds or animal housings are available. In addition we offer the preparation of cells from spleen, blood, bone marrow or lymph nodes for the production of monoclonal antibodies or RNA isolation.

Additional Services for Small Rodent Antibodies
Cat.No. Description Amount Catalog Price
A057Additional Small Bleed-Please ask
A059Additional Immunization1 ImmunizationPlease ask
A705ELISA Raw Data incl. graphFrom 1 Bleed17 EUR
A706Animal Housing1 day, 1 animalPlease ask
N101Spleen Cell Isolation1 spleen172 EUR
N201PBMC Lymphocyte Isolation1 antiserum172 EUR
N301Bone Marrow Cell Isolation1 animal215 EUR
N401Lymph Node Cell Isolation1 animal172 EUR

Order Process

We keep the order process very simple. You can send us your antigen to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The immunizations usually start the same day the antigen arrives. To fasten up the immunization process and help us to connect your antigen with your order ensure you provide the following information:

  1. Your Antigen
  2. Concentration & Buffer Conditions of your Antigen
  3. Our Offer, our order form or your official PO
  4. VAT number (only EU Customers, not German Customers)

If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies

Imprint | Privacy Notice | Contact | Terms & Condition Of Sale | Quality Control

Davids Biotechnologie GmbHemail:info@davids-bio.com
Roentgenstrasse 3phone:+49-941-948228
93055 Regensburgfax:+49-941-9468119
Germany