Please find additional information to the development of monoclonal antibodies including the steps needed and immunization protocols here: Monoclonal Antibody Development.
Davids Biotechnologie develops antibodies since more than 20 years. Our experience leads to flexible methods for our customers. You can start or stop the monoclonal antibody service with each step.
Our monoclonal antibody services include the immunization of 4 mice or 3 rats, the isolation of spleen cells, the fusion with myeloma cells and the screening for positive clones as well as the subcloning of the best clone. You receive protocols to follow the progress of the immunizations and the antibody development. You receive cell culture supernatants from the best clones for your own applications. You reveice the final antibody producing clones.
in vitro monoclonal antibody development can be time saving as no immunization from mice or rat is necessary. For this reason the in vitro development is superior for self-antigens and toxic antigens. The spleen cells are prepared with your antigen for the fusion. Afterwards the spleen cells are fused with the myeloma cells. This method is animal friendly and leads with some antigens to better results than the conservative method. This service includes all steps needed to receive the antibody producing clone:
We produce and purify your antibodies from your antibody producing clone or from a clone we developed for you.
Production from few mg up to several g are possible.
With our additional services we offer you to customize your monoclonal antibody projects to reveice the best antibody against your antigen.
Cat.No. | Description | Amount | Price |
---|---|---|---|
A057 | Additional TestBleed | Depends on animal | on demand |
A705 | Additional ELISA raw data | from 1 serum | Price List |
N101 | Spleen Cell Isolation | 1 Spleen | Price List |
N201 | PBMC Lymphocyte Isolation | 1 serum | Price List |
N301 | Bone Marrow Isolation | 1 animal | Price List |
N401 | Lymph Node Isolation | 1 animal | Price List |
M006 | Subclass Determination IgM/IgG | 1 Clone | Price List |
M008 | Storage of one cell line for one year | 1 Clone, 3 Vials | Price List |
M302 | Mycoplasm Test (PCR) | 1 Cell Culture | Price List |
- | Sterile Filtration | - | on demand |
- | Development of ELISA Methods | - | on demand |
- | Immuno-Histo-Chemistry Analysis | - | on demand |
- | Increase the concentration of IgG | - | on demand |
- | Aliquoting of the product in sevaral vials | - | on demand |