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Antibody Production in Germany, Europe

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Monoclonal Antibody Production

Hybridoma Production and Purification Service

Production and purification of antibodies from hybridoma cells (antibody generating clones) and cell culture supernatants. Please use Davids Monoclonal Development Service or give us your hybridoma or cell culture supernatants for the production and purification.


Antibody Production


Antibody Purification

Transient Transfection

Transient Transfection


Additional Services


Order Process

Antibody Production from Hybridoma

Davids produces and purifies antibodies from your hybridoma cell lines that generate antibodies. You can either send your hybridoma (clone) to Davids for production or utilize one that Davids already created for you previously (Monoclonal Antibody Development).

MAb Production by Davids

Davids exclusively utilizes spinner and roller flasks in in-house built bio reactors for production. The final product is high purity antibody fractions with minimal endotoxin levels. We have a large output range spanning from 10 mg to 10000 mg. Simply request your customized deal for large-scale projects. Table 1 illustrates some examples of antibody production and purification services. In addition, we offer the production in serum-free medium as well as the generation and purification of IgM or other antibodies that do not attach to ProteinA material.

Monoclonal Antibody Production
Production (5 - 10 mg) Production (30 mg) Production (100 mg)
Antibody Production in Cell CultureExpression of ProteinsProduction of Monoclonal Antibodies
We needYour clone frozen (-80°C)Your clone frozen (-80°C)Your clone frozen (-80°C)
Growing3 weeks3 weeks3 weeks
Production8 weeks10 weeks13 weeks
Purification1 weeks1 weeks2 weeks
PurificationProteinA includedProteinA includedProteinA included
Targeted Amount5 - 10 mg30 mg100 mg
Quality ControlDetermination of Protein ConcentrationDetermination of Protein ConcentrationDetermination of Protein Concentration
Mycoplasm Test+++
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Antibody Production Schedule

Please bear in mind that the schedule and yield are dependent on the clone generating the antibody. The time is calculated for clones with a yield of 10 mg/l. Please inquire about special pricing for bigger or smaller volume production. Furthermore, ProteinA does not operate with every clone. Please check our antibody purification services for these clones.

Is the Hybridoma really monoclonal (subclone)?

Verify that the clone has been subcloned. Clones that have not been subcloned may not be monoclonal and, as a result, lose production rate or activity over time. Please utilize our subcloning service if you are unsure whether the clone is genuinely monoclonal.

Purification of Supernatants

Our production services cover the purification with ProteinA or other materials of your supernatants from antibody generating clones. In special cases, further polishing processes are required to remove polymers or other undesirable compounds. We propose polishing steps after ProteinA as an answer for this.
We provide ProteinA independent purification for IgM subclasses or antibodies that do not bind to ProteinA. This approach is used to purify IgM or small antibody fragments, among other things.

Monoclonal Antibody Purification
ProteinA Independent Purification Polishing
Purification of AntibodiesAntibody PurificationAntibody Purification
Schedule1 weeks1 - 2 weeks1 - 2 weeks
Available forIgGIgG
Fragmented Antibodies
Fragmented Antibodies
MethodProteinAIon-Exchange-Chromatography (IEX)
Size-Exclusion-Chromatography (SEC)
Ion-Exchange-Chromatography (IEX)
Size-Exclusion-Chromatography (SEC)
AmountWe are able to purify small and large amounts
Quality ControlProtein ConcentrationProtein ConcentrationProtein Concentration
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Transient Transfection

Transient transfection of proteins and antibodies in suspension cultures of CHO cell cultures provides the benefit of appropriately posttranslationally modified proteins. Protein yields range from 1 to 200 mg per liter of medium. You are welcome to send us your transient transfection plasmid and we generate your protein. You may also send us your plasmid in modest amounts (50 ng) and we will make your plasmid for transient transfection.

Transient Transfection
Plasmid Amplification Transient Transfection in CHO General Transient Transfection
Plasmid Production and IsolationProtein ExpressionProtein Expression
We need50 ng of your transient transfection plasmid1 - 5 mg Plasmid1 - 5 mg Plasmid
Schedule5 days4 - 8 weeks4 - 8 weeks
Purification Production with reduced Endotoxin ProteinA
Other Purifications Available
Other Purifications Available
Quality ControlAgarose GelSDS-PAGE & Protein ConcentrationSDS-PAGE & Protein Concentration
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Additional Services

Additional Services for Monoclonal Antibody Production
Cat.No. Description Amount Catalog Price
M006Subclass Determination: IgG/IgM1 clonePrice List
M008Storage of one cryoconserved clone: 3 vials, 1 year1 clone, 1 yearPrice List
-Sterile Filtration1 fraction
B001ELISA Development: Antigen1 clone
B002ELISA Development: Primary Antibody1 clone
-WesternBlot Analysis1 clone
-Immuno-Histo-Chemistry Analysis1 clone
-Up-Concentration of IgG1 fraction

Order Process & Clone Shipping

We keep the order process very simple. You can send us your clone or supernatant to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg

We start with the production and purification as soon as your material arrives. Please add the following information to help us connect your order with the material:

  1. Type of antibody
  2. Species (mouse, rat, guineapig, ...), Subclass (IgG, IgM, ...)
  3. Is the antibody subcloned
  4. If known type of purification (ProteinA, ProteinG, Independent)
  5. Our Offer, our order form or your official PO
  6. VAT number (only EU customers, not required for German customers)

You have the choice to send us your clone or use one of your clones developed or stored at Davids. You can ship your clone according to the table:

Antibody Producing Clone Shipping
Frozen cellsFreeze the cells with 5 - 10% DMSO or 5% glycerin. You may add 10 - 20% serum.
Cell culturesCentrifuge the cells in log-phase (max. 300 x g), discard the supernatant and resuspend the pellet in fresh medium. Close the lid and send it in the vial together with a non-frozen ice pack.