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Rabbit Antibodies
Polyclonal Rabbit Antisera
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Rabbit Antibody Purification
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Polyclonal Antibody Production in Rabbits

We use New Zealand white rabbits for the production of your polyclonal rabbit antibodies. It is the most used species to produce custom polyclonal antibodies and antisera. You receive a good amount of high-titer and high-affinity antibodies from the atiserum at low costs with rabbits. In addition rabbits are easy to handle and additional serum collections are easy to prepare. The final amount of antiserum is very good comparing with mice, rats or guinea pigs. We offer every step from the antigen to your final antibody. We help you with the decision which kind of antigen is most suitable for your project and what kind of purification you need.
Antigen Preparation

Antigen Requirements


Immunization Schedules

Antibody Purification

Available Purifications

Additional Services

Additional Services


Order Process

Antigen Requirements

You have the choice to use one of the following antigens for the production of your antisera(Table 1).
When you have the purified (native) protein available it is usually the first choice. You can send your purified protein at -20°C or -80°C to Davids. We start the immunizations as soon as your antigen arrives. For proteins that are hard to purify you can use our SDS-gel extraction service. In this case you load your protein to multiple bands of an SDS-gel and cut your specific gel band. Send the gel band at room temperature to Davids. Of course you can use our protein expression service in E. coli when you don't have time to express and purify your protein for the antibody production.
For hard to express proteins or proteins you only know the sequence from we are able to synthesize peptides that represent your protein. These peptides are conjugated to a carrier to improve the antigenicity in front of the immunization process.
In addition Davids offers the preparation of whole cells, virus particles, DNA or small molecules for the immunization. These services complete the antigen services. Though if you have another antigen or when your antigen doesn't fit the requirements in the Table 1, you can contact us: info@davids-bio.com. For further information click on the desired antigen in the table.

Antigens for Rabbit Antibody Production
Antigen Amount Instructions Shipping
Protein0.2 - 0.6 mg
0.3 - 2 mg/ml
We use your protein for the immunization-20°C (ice packs) to -80°C (dry-ice) or lyophilized
Protein Expression-We produce your antigenYou can ship your expression plasmid at -20°C
Protein (SDS-Gel)0.2 - 0.6 mg
3 - 8 gel bands
Cut the gel bands from your protein out of the gelWithout addition of solutions at room temperature
Peptides1 - 2 mg
0.3 - 2 mg/ml
We can synthesize and conjugate your peptides or use your onesLyophilized or with ice packs
Cells106 cfu / immunizationUse our cell immunization kit to prepare for immunizationsPlease contact us when you want to send cells
DNA1 - 2 mg
0.3 - 2 mg/ml
We prepare your DNA for the immunizationsAt room temperature or with ice packs
Small Molecules1 - 5 mg
0.3 - 2 mg/ml
We prepare your small molecules for the immunizationPlease contact us when you want to send small molecules
For Affinity Purification we need additional >0.5 mg protein, 3 mg peptide or 108 cfu

Immunization Schedules

Comparison of titer development after immunization We offer different immunization protocols for your special needs. In addition to our well established immunization schedules we are able to follow your individual one.
Our 63-day Protocol includes 5 immunizations. After the third one (day 35) we take a test bleed and perform an ELISA titer determination. You are able to receive an aliquot of the test bleed and the results of the ELISA titer determination. After day 63 you receive the antiserum or we continue with a purification of the antiserum.

Our 28-day SuperFast Immunization Protocol uses superior adjuvants and an optimized immunization schedule. The titers and antisera are comparable with our 63-day program (see picture).

We start the immunizations as soon as the antigen arrives, usually at the same day. Table 2 shows an overview of our most popular polyclonal rabbit antibody services.

Immunization Protocols
SuperFast Well-Established LowDose Phospho-Specific
Cat.NoA055 (1 rabbit)A053 (1 rabbit)
A054 (2 rabbits)
A056 (1 rabbit)A060 (1 rabbit)
A061 (2 rabbits)
Schedule ProlongationNot PossiblePossibleNot PossiblePossible
Immunizations453 - 55
Test Bleed-day 35day 35day 35
Antiserum Amount40 - 90 ml40 - 90 ml40 - 90 ml-
Purification Options
PurificationPrecipitation (R001)
Affinity Purification (R007)
ProteinA (R008)
Precipitation (R001)
Affinity Purification (R007)
ProteinA (R008)
Precipitation (R001)
ProteinA (R008)
Affinity Purification included
Depletion included
Antigen Requirements
Suitable AntigensProtein
Protein (SDS-Gel)
Small Molecules
Protein (SDS-Gel)
Small Molecules
Protein (SDS-Gel)
Small Molecules
Antigen Needed0.2 - 0.6 mg Protein0.2 - 0.6 mg Protein < 0.3 mg Protein10 mg Peptide (we can synthesize it)
Antigen Concentration0.2 - 0.6 mg/ml0.2 - 0.6 mg/ml0.1 - 0.6 mg/ml1 - 10 mg/ml
Antigen BufferNon toxic bufferNon toxic bufferNon toxic bufferNon toxic buffer
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Our Immunization Schedules are well established and optimized for most customers. Though we are able to follow your immunization protocol or modify ours with additional intermediate bleeds, longer and additional immunizations and additional ELISA titer determinations.

Comparison of Immunization Schedules
Day 63 Day Antisera
1Preimmune serum, 0.5 ml
11. Immunization
142. Immunization
283. Immunization
  • Test serum, 1ml
  • ELISA-Titer determination
  • Optional: Test serum for the customer
424. Immunization
565. Immunization
63Antiserum, 40 - 90 ml
Get your offer to extend animal housing and the number of immunizations.
Day 28 Day SuperFast Antisera
1Preimmune serum, 1 ml
11. Immunization
72. Immunization
143. Immunization
214. Immunization
28Anti serum: 40 - 90 ml
Day Custom Protocol
XPreimmune serum, 1 ml
ZIntermediate Bleed
AAntiserum: 40 - 90 ml

Rabbit Antiserum Purification

We offer different purification services for your polyclonal rabbit antibodies. Most common is the affinity purification, which we recommend for many applications like WesternBlot or IHC, to receive specific antibodies that only recognize your antigen. This service is available for Proteins, Peptides and other antigens.
In addition we offer ProteinA purifications to receive a pure IgG fraction or depletions to get rid of unwanted antibodies that i. e. recognize tags.

Purification Options for Polyclonal Rabbit Antibodies
Affinity Purification ProteinA Cell Culture Purity Depletion
Cat.NoR007 (1 antiserum)
R007-2 (2 antisera)
R008 (1 antiserum)R003 (1 antiserum)R101 (anti-tag)
R102 (anti-E. coli)
Duration5 days5 days5 - 10 days1 - 5 days
Product Information
ColumnAntigen bound to resinProteinA bound to resinAntigen bound to resintag/E. coli bound to resin
ProductSpecific AntibodiesWhole IgG FractionSpecific Antibodies
Low Endotoxin
Fraction without unwanted Antibodies
Amount (approximately)0.5 - 10 mg5 - 30 mg0.1 - 10 mgDepends on Depleted Fraction
Optimal forWesternBlot
ImmunoHistoChemistry (IHC)
Fluorescent Activated Cell Sorting (FACS)
ImmunoHistoChemistry (IHC)
in vivo Applications
Fluorescent Activated Cell Sorting (FACS)
Depends on Depleted Fraction
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Additional Services

We offer to determine the preimmune reaction of multiple rabbits with your antigen. Here we are able to immunize a rabbit that shows only a small immune reaction to your antigen. Within the immunization schedule we are able to take additional small (0.5 ml) or large bleeds (15 ml) for ELISA determinations or for your applications.

Additional Services for Polyclonal Rabbit Antibodies
Cat.No. Description Amount Catalog Price
A057Additional Small Bleed0.5 - 1 ml23 EUR
A058Additional Large Bleed15 ml60 EUR
A059Additional Immunization1 Immunization17 EUR
A070Preimmune Selection for the immunization of 1 Rabbit3 Preimmune55 EUR
A705ELISA Raw Data incl. graphFrom 1 Bleed17 EUR
A706Animal Housing1 day, 1 rabbit2.90 EUR
N101Spleen Cell Isolation1 spleen172 EUR
N201PBMC Lymphocyte Isolation1 antiserum249 EUR
N301Bone Marrow Cell Isolation1 rabbit215 EUR
N401Lymph Node Cell Isolation1 rabbit172 EUR

Order Process

We keep the order process very simple. You can send us your antigen to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg

The immunizations usually start the same day the antigen arrives. To fasten up the immunization process and help us to connect your antigen with your order ensure you provide the following information:

  1. Your Antigen
  2. Concentration & Buffer Conditions of your Antigen
  3. Our Offer, our order form or your official PO
  4. VAT number (only EU Customers, not German Customers)

If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies

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Davids Biotechnologie GmbHemail:info@davids-bio.com
Roentgenstrasse 3phone:+49-941-948228
93055 Regensburgfax:+49-941-9468119