Proteins in its native or denaturated forms are usually the antigen of choice when the protein is available. The immunogenicity is in most cases good as multiple epitopes are available on the protein. In addition the accessibility of the final antibodies to your antigen in your applications is very high when you use the same antigen. The immunization can be done with any purity. Though the chances are high that antibodies are generated against the impurities. Consider to run a SDS-gel and send us the antigen specific gel bands as antigen when your purity is low.
For tagged proteins we can perform a depletion when the anti-tag antibodies disturb in your assays.
You can send your whole protein or a part of your protein to Davids Biotechnologie GmbH, Roentgenstrasse 3, 93055 Regensburg, Germany. Davids starts with the immunizations as soon as your antigen arrives. Please have a look at our buffer requirements: Buffer Requirements before you send the antigen. For tagged antigens please have a look at our additional information.
Proteins can usually be send with ice packs, lyophilized or on dry ice.
|For Immunizations (1 animal)||0.5 mg||0.5 mg||20 - 250 µg||20 - 250 µg||0.15 - 0.5 mg|
|For Affinity Matrix||0.5 - 2 mg||0.5 - 2 mg||0.5 - 2 mg||0.5 - 2 mg||0.5 - 2 mg|
|Recommended Concentration||0.3 - 2 mg/ml||0.3 - 2 mg/ml||0.3 - 2 mg/ml||0.3 - 2 mg/ml||0.3 - 2 mg/ml|
|Buffer||Any physiological non-toxic buffer like 1 x PBS or 0.9% NaCl. For more information: buffer recommendations|
Gel pieces from SDS-gels are easy to use and have some advantages. You save time with complicated purification steps. In addition you are able to get rid of even small protein contaminations.
The gel extraction is simple. For example: Prepare a solution with at least 0.5 mg protein for the immunization of one rabbit and additional 0.5 mg protein if you want to order an affinity purification. Just run multiple bands with your protein solution, dye the gel with coomassie and cut all bands that belongs to your protein from the SDS-PAGE gel. Send the gel pieces in a 2-ml or 15-ml tube at room temperature without the addition of any solvents to Davids Biotechnologie GmbH, Roentgenstrasse 3, 93055 Regensburg, Germany.
The final antibodies are usually very good for applications like western blot and all other fields where you need to detect the denaturated protein. Even though for some applications where you need to detect the protein in its native form the immunization of the native protein has advantages.
When your protein is not available you can use our peptide synthesis service. For Peptide Antibodies the peptide represents the full length protein. Davids helps you to detect peptides with a good predicted antigenicity, solubility and epitopes.
When the expression plasmid is available, the expression of your protein for the antibody production is an easy way to receive the full length protein. Just use our expression service. If you have other antigens just have a look at our antigen overview or write us an email with personal suggestions: firstname.lastname@example.org.
Purified proteins with tags as antigen leads to an antibody fraction with antibodies recognizing the protein and the tag. When these anti-tag antibodies disturb in your assays, there are multiple ways to remove these antibodies. The depletion service Cat.No.R101 removes the anti-tag antibodies with Davids tag-depletion chromatography.
If you order an antigen specific affinity purification the removal of the anti-tag antibodies can be done when a protein without a tag or with another tag for the affinity purification is used. I. e. we use a HIS-tagged protein for the immunization and a non-tagged or STREP-tagged protein for the affinity purification.
For proteins smaller than 10 kDa we recommend to perform a conjugation with your protein. The conjugation of the protein activates the immune system of the animals in its best way. For more information please go to our conjugation services.
We produce antibodies from antigens with any purity. Please keep in mind that antibodies against all impurities are made as well. To receive specific antibodies even with less pure proteins we recommend our SDS-gel extraction service or a subsequent affinity purification with pure protein.
Davids keeps the order process very simple. You can send your antigen to:
Davids Biotechnologie GmbH
The immunizations usually start at the same day the antigen arrives. To fasten up the immunization process and help us to connect your antigen with your order ensure you provide the following information:
If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies