Polyclonal Antibody Production in Mice, Rats and Guinea pigs

All animals are housed in our department. This ensures animal friendly and cost efficient housings. We are able to have individual looks on each animal, the immunization schedules and your specific needs. We use BALB/c mice and black hooded rats for your antibody production. To complete our services for small rodents we offer guinea pigs as well. The advantage of rats and guinea pigs are the larger amount of antiserum from one animal.
For monoclonal projects please go to our Monoclonal Development Page.

Antigen Requirements

Immunization Schedules

Available Purifications

Additional Services

Order Process

Antigen Requirements

For optimal immunization success we offer to immunize multiple mice, rats or guinea pigs with different antigen concentrations to receive best results. The antigen amount needed for one animal are listed in the table below. Depending which kind of antigen you choose we need different amount of antigen. We already successfully generated antibodies with even less amount of the recommended antigen amount. If you have less antigen please contact us: info@davids-bio.com. For further information click on the desired antigen.

Antigens for Small Rodent Antibody Production

	Antigen	1 Mouse	1 Rat	1 Guinea pig	Description
	Protein	20 - 250 µg 0.3 - 2 mg/ml	20 - 250 µg 0.3 - 2 mg/ml	0.15 - 0.5 mg 0.3 - 2 mg/ml	We use your protein for the immunization
	Protein Expression	-	-	-	We produce your antigen from your plasmid
	Protein (SDS-Gel)	25 - 250 µg 3 - 8 gel bands	0.15 - 0.3 mg 3 - 8 gel bands	0.15 - 0.6 mg 3 - 8 gel bands	Cut your specific protein band from your SDS-gel
	Peptides	1 - 2 mg 0.3 - 2 mg/ml	1 - 2 mg 0.3 - 2 mg/ml	1 - 2 mg 0.3 - 2 mg/ml	Use our synthesis and/or conjugation service
	Cells	106 cfu / immunization	106 cfu / immunization	106 cfu / immunization	Please contact us when you want to use cells as antigen
	DNA	1 - 2 mg 0.3 - 2 mg/ml	1 - 2 mg 0.3 - 2 mg/ml	1 - 2 mg 0.3 - 2 mg/ml	We prepare your DNA for the immunizations
	Small Molecules	1 - 5 mg 0.3 - 2 mg/ml	1 - 5 mg 0.3 - 2 mg/ml	1 - 5 mg 0.3 - 2 mg/ml	We prepare your small molecules for the immunizations
For Affinity Purification we need additional >0.5 mg protein, 3 mg peptide or 108 cfu

Immunization Schedules for Small Rodents

Our well established 63-day immunization schedule offers the best results in titer development. For your special needs we are able to follow your immunization protocols or immunization techniques. Longer immunization times, additional immunizations, additional test bleeds or special immunization techniques are available. We can immunize large amounts of animals. For large projects with mouse, rats or guinea pigs please ask for your special offer: info@davids-bio.com.

Immunization Protocols

	Mouse	Rat	Guinea Pig
Cat.No	A401 (1 mouse) A402 (2 mice) A404 (4 mice)	A205 (1 rat) A206 (2 rats)	A201 (1 guinea pig) A202 (2 guinea pigs)
Schedule	63-day	63-day	63-day
Schedule Prolongation	Possible	Possible	Possible
Immunizations	5	5	5
Titer Determination	day 35	day 35	day 35
Serum Amount	0.2 - 1 ml	2 - 10 ml	5 - 15 ml
Purification Options
Purification	Precipitation (R001) Affinity Purification (R007) ProteinA (R008)	Precipitation (R001) Affinity Purification (R007) ProteinA (R008)	Precipitation (R001) ProteinA (R008)
Antigen Requirements
Suitable Antigens	Protein Protein (SDS-Gel) Peptide DNA Cells Small Molecules	Protein Protein (SDS-Gel) Peptide DNA Cells Small Molecules	Protein Protein (SDS-Gel) Peptide DNA Cells Small Molecules
Antigen Needed	20 - 250 µg Protein	20 - 250 µg Protein	0.15 - 0.5 mg Protein
Antigen Buffer	Non toxic buffer	Non toxic buffer	Non toxic buffer
Price	Price List	Price List	Price List
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Rodent Immunization Schedules

Day	63 Day Antisera
1	Preimmune serum
1	1. Immunization
14	2. Immunization
28	3. Immunization
35	Test serum ELISA-Titer determination Optional: Test serum for the customer
42	4. Immunization
56	5. Immunization
63	Anti serum
∞	Get your offer to extend animal housing and the number of immunizations.

Antisera Purification

We offer different purification services for your polyclonal antisera from mouse, rat and guinea pig. We recommend to pool antisera from multiple mice before the purification to enhance the yield. Separate purifications from single antisera are available as well.
You have the choice of an affinity purification for specific antibodies and ProteinA purifications to receive a pure IgG fraction or depletions to get rid of unwanted antibodies that i. e. recognize tags.

Purification Options for Polyclonal Antibodies from Mice, Rats and Guinea Pigs

	Affinity Purification	ProteinA	Cell Culture Purity	Depletion
Cat.No	R007 (1 antiserum pool) R007-2 (2 antisera)	R008 (1 antiserum pool)	R003 (1 antiserum pool)	R101 (anti-tag) R102 (anti-E. coli)
Duration	5 days	5 days	5 - 10 days	1 - 5 days
Product Information
Column	Antigen bound to resin	ProteinA bound to resin	Antigen bound to resin	tag/E. coli bound to resin
Product	Specific Antibodies	Whole IgG Fraction	Specific Antibodies Low Endotoxin Sterile	Fraction without unwanted Antibodies
Applications
Optimal for	WesternBlot ImmunoHistoChemistry (IHC) Fluorescent Activated Cell Sorting (FACS) ELISA	ImmunoHistoChemistry (IHC)	in vivo Applications Fluorescent Activated Cell Sorting (FACS)	Depends on Depleted Fraction
Catalog Price	Price List	Price List	Price List	Price List
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Additional Services

We offer multiple additional services for your special needs. Additional Immunizations, Test bleeds or animal housings are available. In addition we offer the preparation of cells from spleen, blood, bone marrow or lymph nodes for the production of monoclonal antibodies or RNA isolation.

Additional Services for Small Rodent Antibodies

Cat.No.	Description	Amount	Catalog Price
A057	Additional Small Bleed	-	Please ask
A059	Additional Immunization	1 Immunization	Please ask
A705	ELISA Raw Data incl. graph	From 1 Bleed	17 EUR
A706	Animal Housing	1 day, 1 animal	Please ask
N101	Spleen Cell Isolation	1 spleen	172 EUR
N201	PBMC Lymphocyte Isolation	1 antiserum	172 EUR
N301	Bone Marrow Cell Isolation	1 animal	215 EUR
N401	Lymph Node Cell Isolation	1 animal	172 EUR

Order Process

We keep the order process very simple. You can send us your antigen to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The immunizations usually start the same day the antigen arrives. To fasten up the immunization process and help us to connect your antigen with your order ensure you provide the following information:

1. Your Antigen
2. Concentration & Buffer Conditions of your Antigen
3. Our Offer, our order form or your official PO
4. VAT number (only EU Customers, not German Customers)

If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies