Polyclonal Antibody Production in Mice, Rats and Guinea pigs

The animals are housed in our own animal department in Germany (Europe). This ensures animal friendly and cost efficient antibody production. Frequently individual observing of each animal and the titer development ensures high qulity antibodies with best titers. BALB/c mice and black hooded rats are used for the antibody production. To complete our services for small rodents the immunization of guinea pigs is available. The advantage of rats and guinea pigs are the larger amount of antiserum from one animal.
For monoclonal projects please go to our Monoclonal Development Page.

Antigen Requirements

Immunization Schedules

Available Purifications

Additional Services

Order Process

Antigen Requirements

For optimal immunization success the immunization of multiple mice, rats or guinea pigs with different antigen concentrations is recommended to receive best results. The average antigen amount needed for the immunization of one animal is listed in the table below. Depending which kind of antigen you choose, the amount of antigen might be different. Davids already successfully generated antibodies with even less amount of the recommended antigen . If you have less antigen please contact us: info@davids-bio.com. For further information click on the desired antigen.

	Antigen	1 Mouse	1 Rat	1 Guinea pig	Description
	Protein	100 - 250 µg 0.3 - 2 mg/ml	100 - 250 µg 0.3 - 2 mg/ml	200 - 500 µg 0.3 - 2 mg/ml	Customer Provided Proteins
	Protein Expression	-	-	-	Protein Expression from your plasmid
	Protein (SDS-Gel)	150 - 250 µg 3 - 8 gel bands	150 - 300 µg 3 - 8 gel bands	200 - 600 µg 3 - 8 gel bands	Protein Extraction from SDS-gel pieces. Cut the protein specific gel bands from the gel.
	Peptides	1 - 2 mg 0.3 - 2 mg/ml	1 - 2 mg 0.3 - 2 mg/ml	1 - 2 mg 0.3 - 2 mg/ml	Customer provided peptides or Peptide synthesis and Conjugation Service by Davids.
	Cells	5 x 10 cfu	5 x 107 cfu	5 x 107 cfu	Preparation with Davids Cell Immunization KIT for optimal presentation of the surface to the immune system.
	DNA	1 - 2 mg 0.3 - 2 mg/ml	1 - 2 mg 0.3 - 2 mg/ml	1 - 2 mg 0.3 - 2 mg/ml	DNA preparation for the immunizations by Davids
	Small Molecules	1 - 5 mg 0.3 - 2 mg/ml	1 - 5 mg 0.3 - 2 mg/ml	1 - 5 mg 0.3 - 2 mg/ml	Preparation of small molecules by Davids
For Affinity Purification we need additional 0.5 - 2 mg protein, 3 mg peptide or 108 cfu

Immunization Schedules for Small Rodents

Our well established 63-day immunization schedule offers the best results in titer development and affinity for most antigens. For your special needs we are able to follow your individual immunization protocols or immunization techniques. Longer immunization times, additional immunizations, additional test bleeds or special immunization techniques are available. For large projects with a high number of mice, rats or guinea pigs please ask for your special offer: info@davids-bio.com.

	Mouse	Rat	Guinea Pig
Cat.No	A401 (1 mouse) A402 (2 mice) A404 (4 mice)	A205 (1 rat) A206 (2 rats)	A201 (1 guinea pig) A202 (2 guinea pigs)
Schedule	63-day	63-day	63-day
Schedule Prolongation	Possible	Possible	Possible
Immunizations	5	5	5
Titer Determination	day 35	day 35	day 35
Serum Amount	0.2 - 0.7 ml	2 - 8 ml	5 - 15 ml
Purification Options
Purification	Precipitation (R001) Affinity Purification (R007) ProteinA (R008)	Precipitation (R001) Affinity Purification (R007) ProteinA (R008)	Precipitation (R001) Affinity Purification (R007) ProteinA (R008)
Antigen Requirements
Suitable Antigens	Protein Protein (SDS-Gel) Peptide DNA Cells Small Molecules	Protein Protein (SDS-Gel) Peptide DNA Cells Small Molecules	Protein Protein (SDS-Gel) Peptide DNA Cells Small Molecules
Antigen Needed	100 - 250 µg Protein	100 - 250 µg Protein	200 - 500 µg Protein
Antigen Buffer	Non toxic buffer	Non toxic buffer	Non toxic buffer
Price	Price List	Price List	Price List
Get your free offer now

Antisera Purification

We offer different purification services for your polyclonal antisera from mouse, rat and guinea pig. We recommend to pool antisera from multiple mice before the purification to enhance the yield. Separate purifications from single antisera are available as well.
You have the choice of an affinity purification for specific antibodies and ProteinA purifications to receive a pure IgG fraction or depletions to get rid of unwanted antibodies that i. e. recognize tags.

	Affinity Purification	ProteinA	Cell Culture Purity	Depletion
Cat.No	R007 (1 antiserum pool) R007-2 (2 antisera)	R008 (1 antiserum pool)	R003 (1 antiserum pool)	R101 (anti-tag) R102 (anti-E. coli)
Duration	5 days	5 days	5 - 10 days	1 - 5 days
Product Information
Column	Antigen bound to resin	ProteinA bound to resin	Antigen bound to resin	tag/E. coli bound to resin
Product	Specific Antibodies	Whole IgG Fraction	Specific Antibodies Low Endotoxin Sterile	Fraction without unwanted Antibodies
Applications
Optimal for	WesternBlot Immuno-Histo-Chemistry (IHC) Fluorescent Activated Cell Sorting (FACS) ELISA	Immuno-Histo-Chemistry (IHC)	in vivo Applications Fluorescent Activated Cell Sorting (FACS)	Depends on Depleted Fraction
Catalog Price	Price List	Price List	Price List	Price List
Get your free offer now

Additional Services

We offer multiple additional services for your special needs. Additional Immunizations, Test bleeds or animal housings are available. In addition we offer the preparation of cells from spleen, blood, bone marrow or lymph nodes for the production of monoclonal antibodies or RNA isolation.

Order Process

Davids keeps the order process very simple. Just send your antigen to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The immunizations usually start at the same day the antigen arrives. Please provide the following information to fasten up the start and to help us connect your antigen with the correct order:

1. Your Antigen
2. Concentration & Buffer Conditions of your Antigen
3. Our Offer, our order form or your official PO
4. VAT number (only EU Customers, not German Customers)

If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies