Mouse, Rat and Guinea pig antibody production
The animals are housed in our own animal department in Germany (Europe). This guarantees that antibody manufacturing is both animal-friendly and cost-effective. Individual monitoring of each animal and titer progression on a regular basis assures high quality antibodies with optimal titers. For antibody production, BALB/c mice and black hooded rats are used as a standard. Other strains are available on demand. Guinea pig vaccination is provided to enhance our rodent expertise. Rats and guinea pigs have the benefit of producing a bigger volume of antiserum from a single animal.
Please visit our Monoclonal Development Page for additional information on monoclonal antibody projects.
Antigen Requirements
Learn more about our rodent antigen requirements.
Immunization Schedules
Take advantage of our well-established immunization methods.
Available Purifications
Additional Services
Isolation of cells from spleen and bone marrow as well as DNA preparation
Order Process
Antigen Requirements
To achieve the best results, it is advised that numerous mice, rats, or guinea pigs be immunized with various antigen concentrations. The table below shows the average antigen quantity required for vaccination of one animal. The quantity of antigen may change depending on the type of antigen used. Davids has already successfully produced antibodies using a tiny amount of the required antigen. Please email us at info@davids-bio.com if you have less antigen. Click on the selected antigen for more details.
| Antigen | 1 Mouse | 1 Rat | 1 Guinea pig | Description | |
|---|---|---|---|---|---|
 | Protein | 100 - 250 µg 0.3 - 2 mg/ml | 100 - 250 µg 0.3 - 2 mg/ml | 200 - 500 µg 0.3 - 2 mg/ml | Proteins supplied by customer |
 | Protein Expression | - | - | - | Protein expression from your plasmid |
 | Protein (SDS-Gel) | 150 - 250 µg 3 - 8 gel bands | 150 - 300 µg 3 - 8 gel bands | 200 - 600 µg 3 - 8 gel bands | Protein extraction from SDS-gel pieces. Cut the protein specific gel bands from the gel. |
 | Peptides | 1 - 2 mg 0.3 - 2 mg/ml | 1 - 2 mg 0.3 - 2 mg/ml | 1 - 2 mg 0.3 - 2 mg/ml | Customer provided peptides or peptide synthesis and conjugation service by Davids. |
 | Cells | 5 x 107 - 1 x 108 cfu | 5 x 107 - 1 x 108 cfu | 5 x 107 - 1 x 108 cfu | Preparation with Davids Cell Immunization KIT for optimal presentation of the surface to the immune system. |
 | DNA | 1 - 2 mg 0.3 - 2 mg/ml | 1 - 2 mg 0.3 - 2 mg/ml | 1 - 2 mg 0.3 - 2 mg/ml | DNA preparation for the immunizations by Davids |
 | Small Molecules | 1 - 5 mg 0.3 - 2 mg/ml | 1 - 5 mg 0.3 - 2 mg/ml | 1 - 5 mg 0.3 - 2 mg/ml | Preparation of small molecules by Davids |
| For Affinity Purification we need additional 0.5 - 2 mg protein, 3 mg peptide or 108 cfu | |||||
Immunization Schedules for Small Rodents
Our well-established 63-day vaccination strategy delivers the best results in terms of titer development and antigen affinity for the majority of antigens. We can follow your specific immunization protocols or immunization strategies for your special requirements. There are options for extended immunization schedules, extra vaccinations, further test bleeds, and specific immunization techniques. For large projects including a significant number of mice, rats, or guinea pigs, please contact us at info@davids-bio.com for a special discount.
 Mouse |
 Rat |
 Guinea Pig |
|
|---|---|---|---|
| Cat.No | A402 (2 mice) A404 (4 mice) | A206 (2 rats) | A202 (2 guinea pigs) |
| Schedule | 63-day | 63-day | 63-day |
| Schedule Prolongation | Possible | Possible | Possible |
| Immunizations | 5 | 5 | 5 |
| Titer Determination | day 35 | day 35 | day 35 |
| Serum Amount | 0.2 - 0.7 ml | 2 - 8 ml | 5 - 15 ml |
| Purification Options | |||
| Purification | Precipitation (R001) Affinity Purification (R007) ProteinA (R008) | Precipitation (R001) Affinity Purification (R007) ProteinA (R008) | Precipitation (R001) Affinity Purification (R007) ProteinA (R008) |
| Antigen Requirements | |||
| Suitable Antigens | Protein Protein (SDS-Gel) Peptide DNA Cells Small Molecules | Protein Protein (SDS-Gel) Peptide DNA Cells Small Molecules | Protein Protein (SDS-Gel) Peptide DNA Cells Small Molecules |
| Antigen Needed | 100 - 250 µg Protein | 200 - 500 µg Protein | 200 - 500 µg Protein |
| Antigen Buffer | Non toxic buffer | Non toxic buffer | Non toxic buffer |
| Price | Price List | Price List | Price List |
 Get your free quotation now | |||
| Day | 63 Day Antisera |
|---|---|
| 1 | Preimmune serum |
| 1 | 1. Immunization |
| 14 | 2. Immunization |
| 28 | 3. Immunization |
| 35 |
|
| 42 | 4. Immunization |
| 56 | 5. Immunization |
| 63 | Anti serum |
| ∞ | Get your offer to extend animal housing and the number of immunizations. |
Antisera Purification
We provide various purification services for mouse, rat, and guinea pig polyclonal antisera. To increase yield, we propose pooling antisera from individual mice before purification. Separate purifications from each antisera are also available.
There are affinity purifications for specific antibodies and ProteinA purifications to get a pure IgG fraction, as well as depletions to remove undesired antibodies that identify tags.
| Affinity Purification | ProteinA | Cell Culture Purity | Depletion | |
|---|---|---|---|---|
| Cat.No | R007 (1 antiserum pool) R007-2 (2 antisera) | R008 (1 antiserum pool) | R003 (1 antiserum pool) | R101 (anti-tag) R102 (anti-E. coli) |
Antigen Specific Antibodies: | Whole IgG Fraction: | Antigen Specific Antibodies: | Anti-tag Antibody Removal: | |
Tag:  Antigen Specific Antibody:  Unspecific Antibody:  Tag Antibody:  Antigen:  | ||||
| Duration | 5 days | 5 days | 5 - 10 days | 1 - 5 days |
| Product Information | ||||
| Column | Antigen bound to resin | ProteinA bound to resin | Antigen bound to resin | tag/E. coli bound to resin |
| Product | Specific Antibodies | Whole IgG Fraction | Specific Antibodies Low Endotoxin Sterile | Fraction without unwanted Antibodies |
| Applications | ||||
| Optimal for | WesternBlot Immuno-Histo-Chemistry (IHC) Fluorescent Activated Cell Sorting (FACS) ELISA | Immuno-Histo-Chemistry (IHC) | in vivo Applications Fluorescent Activated Cell Sorting (FACS) | Depends on Depleted Fraction |
| Catalog Price | Price List | Price List | Price List | Price List |
| Get your free offer now | ||||
Additional Services
We provide a variety of supplementary services to meet your specific requirements. There are further immunizations, test bleeds, and animal housings offered. We also provide cell preparation from the spleen, blood, bone marrow, or lymph nodes for the generation of monoclonal antibodies or RNA isolation.
| Cat.No. | Description | Amount | Catalog Price |
|---|---|---|---|
| A057 | Additional Small Bleed | - | Price List |
| A059 | Additional Immunization | 1 Immunization | Price List |
| A705 | ELISA Raw Data incl. graph | From 1 Bleed | Price List |
| A706 | Animal Housing | 1 day, 1 animal | Price List |
| N101 | Spleen Cell Isolation | 1 spleen | Price List |
| N201 | PBMC Lymphocyte Isolation | 1 antiserum | Price List |
| N301 | Bone Marrow Cell Isolation | 1 animal | Price List |
| N401 | Lymph Node Cell Isolation | 1 animal | Price List |
Order Process
Davids keeps the ordering procedure as easy as possible. Please send your antigen to:
Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany
Immunizations usually begin on the same day as the antigen arrives. Please submit the following information to assist us correlate your antigen with your order and accelerate the process:
- Your Antigen
- Concentration & Buffer Conditions of your Antigen
- Our Offer, our order form or your official PO
- VAT number (only EU customers, not required for German customers)
Create your own quote with our Antibody Creator. When you prefer to order peptide antibodies, send us your protein sequence and we will recommend peptides that represent your protein. For further information, please visit: Peptide Antibodies
