Antibody Purification from Antisera and other fractions

Purification of antibodies and proteins from antisera, egg yolk, supernatants and other antibody fractions. Purification of material from your lab or antisera that were produced by Davids with your antigen.

Antisera Purifications

Egg Yolk Purifications

Supernatant Purification

Order Process

Antibody Purification from Antisera

Purification of antisera is done for better results in your applications. Applications like WesternBlot, IHC, DotBlot or ELISA profit by purification of the antisera. The antibody fraction is for example high specific (antigen specific affinity purification) or high pure (Whole IgG ProteinA purification). The table shows our most ordered purification methods, though we purify larger amounts of antisera, supernatants and egg yolks as well. Just ask for your special offer. For our antibody production services including the immunizations go to our Rabbit or Small Rodent (Mice, Rats, Guinea pigs) section.

	Affinity Purification	ProteinA	Cell Culture Purity	Depletion
Cat.No	R007 (10 - 50 ml) R007-2 (50 - 100 ml)	R008 (10 - 50 ml)	R003 (10 - 50 ml)	R101 (anti-tag) R102 (anti-E. coli)
Duration	5 - 10 days	5 days	5 - 10 days	5 days
Product Information
Column	Antigen bound to resin	ProteinA bound to resin	Antigen bound to resin	tag/E. coli bound to resin
Additional Information	Please send your antigen (0.5 - 1 mg) for the creation of the affinity matrix		Please send your antigen (0.5 - 1 mg) for the creation of the affinity matrix	Optional: Send the fraction for purification
Product	Specific Antibodies	Whole IgG Fraction	Specific Antibodies Low Endotoxin Sterile	Fraction without unwanted Antibodies
Applications
Optimal for	WesternBlot ImmunoHistoChemistry (IHC) Fluorescent Activated Cell Sorting (FACS) ELISA	ImmunoHistoChemistry (IHC)	in vivo Applications Fluorescent Activated Cell Sorting (FACS)	Depends on Depleted Fraction
Price	Price List	Price List	Price List	Price List
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Antibody Purification from Egg Yolk

The preparation of egg yolks to receive the antibodies is one of the most important steps to receive the best chicken antibodies. Davids Preparation I removes most lipids and lipoproteins and can be used directly in most applications. Preparation II removes remaining lipids and lipoproteins and make a polishing step by chromatography. Preparation III performs an affinity purification at a low endotoxin level and sterile filtration for direct use in cell culture. In addition the antigen specific affinity purifications for your egg yolk antibodies is recommended for most applications and can be performed after Preparation I. For our chicken egg yolk antibody services including the immunizations go to our Chicken Egg Yolk Antibody Section.

	PrepI	PrepII	PrepIII	Affinity Purification
Cat.No	R001	R003	R006	R017
Duration	5 days	5 days	5 - 10 days	1 - 5 days
Product Information
Procedure	Preparation	Chromatography	Antigen bound to resin	Antigen bound to resin
Comparable with	Raw Serum	ProteinA	PrepIII	Affinity Purification
Product	Raw Antibodies in buffer	High purity, raw antibodies	Specific Antibodies Low Endotoxin Sterile	Specific Antibodies
Purity	70 - 90%	90 - 98%	Antigen Specific	Antigen Specific
Amount (approx.)	10 eggs corresponds to 100 ml Serum	10 eggs corresponds to 100 ml Serum	0.1 - 10 mg (10 eggs)	0.5 - 10 mg (10 eggs)
Applications
Optimal for	ELISA	Immuno-Histo-Chemistry (IHC)	in vivo Applications Fluorescent Activated Cell Sorting (FACS)	WesternBlot Immuno-Histo-Chemistry (IHC) Fluorescent Activated Cell Sorting (FACS) ELISA
Price	Price List	Price List	Price List	Price List
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Purification of Supernatants from Monoclonal Antibodies

Davids offers the purification of your monoclonal antibody supernatants or the production & purification of your monoclonal antibody producing clone/hybridoma. Please have a look at our Monoclonal Antibody Production & Purification section. Alternatively you can leave the antibody development to us with our Monoclonal Antibody Development Service.

Order Process

We keep the order process very simple. You can send us your material to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The purification usually starts as soon as your material arrives. To fasten up the process and help us to connect your material with your order ensure you provide the following information:

1. Your Antigen for affinity purifications only
2. Your Antibody containing solution
3. Your desired Purification
4. Our Offer, our order form or your official PO
5. VAT number (only EU Customers, not German Customers)