Purification

Davids Offers the purification of antibodies and proteins from antisera, egg yolk and supernatants. We are able to purify material from your lab or antibodies that were produced at Davids.

Antisera Purifications

Egg Yolk Purifications

Supernatant Purification

Order Process

Purification of Antisera

Purification of antisera is done for better results in your applications. Applications like WesternBlot, IHC, DotBlot or ELISA profit for purifications of the antisera. In addition you receive a pure antibody fraction depending which kind of purification you choose. The table shows our most ordered products, though we purify larger amounts of antisera, supernatants and egg yolks as well. Just ask for your special offer. For our antibody production services including the immunizations go to our Rabbit or Small Rodent (Mice, Rats, Guinea pigs) section.

Antisera Purification

	Affinity Purification	ProteinA	Cell Culture Purity	Depletion
Cat.No	R007 (10 - 50 ml) R007-2 (50 - 100 ml)	R008 (10 - 50 ml)	R003 (10 - 50 ml)	R101 (anti-tag) R102 (anti-E. coli)
Duration	5 - 10 days	5 days	5 - 10 days	5 days
Product Information
Column	Antigen bound to resin	ProteinA bound to resin	Antigen bound to resin	tag/E. coli bound to resin
Additional Information	Please send your antigen (0.5 - 1 mg) for the creation of the affinity matrix		Please send your antigen (0.5 - 1 mg) for the creation of the affinity matrix
Product	Specific Antibodies	Whole IgG Fraction	Specific Antibodies Low Endotoxin Sterile	Fraction without unwanted Antibodies
Applications
Optimal for	WesternBlot ImmunoHistoChemistry (IHC) Fluorescent Activated Cell Sorting (FACS) ELISA	ImmunoHistoChemistry (IHC)	in vivo Applications Fluorescent Activated Cell Sorting (FACS)	Depends on Depleted Fraction
Price	Price List	Price List	Price List	Price List
Get your offer with special prices now

Purification of Egg Yolk

We purify your egg yolk antibodies. Preparation I removes most lipids and lipoproteins. Preparation II removes remaining lipids and lipoproteins and make a polishing step. Preparation III performs an affinity purification at a low endotoxin level and sterile filtration for direct use in cell culture. In addition we offer affinity purifications for your egg yolk antibodies. For full chicken egg yolk antibody services including the immunizations go to our Chicken Egg Yolk Antibody Section.

Purification Options for Chicken Egg Yolk Antibodies

	PrepI	PrepII	PrepIII	Affinity Purification
Cat.No	R001	R003	R006	R017
Duration	5 days	5 days	5 - 10 days	1 - 5 days
Product Information
Procedure	Preparation	Chromatography	Antigen bound to resin	Antigen bound to resin
Comparable with	Raw Serum	ProteinA	PrepIII	Affinity Purification
Product	Raw Antibodies in buffer	High purity, raw antibodies	Specific Antibodies Low Endotoxin Sterile	Specific Antibodies
Purity	70 - 90%	90 - 98%
Amount (approx.)	8 eggs corresponds to 100 ml Serum	8 eggs corresponds to 100 ml Serum	0.1 - 10 mg (8 eggs)	0.5 - 10 mg (8 eggs)
Applications
Optimal for	ELISA	Immuno-Histo-Chemistry (IHC)	in vivo Applications Fluorescent Activated Cell Sorting (FACS)	WesternBlot Immuno-Histo-Chemistry (IHC) Fluorescent Activated Cell Sorting (FACS) ELISA
Price	Price List	Price List	Price List	Price List
Get your offer with special prices now

Purification of Supernatants from Monoclonal Antibodies

We offer the purification of your monoclonal antibody supernatants or the production & purification of your monoclonal antibody producing clone. Please have a look at our Monoclonal Antibody Production & Purification section. Alternatively you can leave the antibody development to us with our Monoclonal Antibody Development Service.

Order Process

We keep the order process very simple. You can send us your material to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The purification usually starts as soon as your material arrives. To fasten up the process and help us to connect your material with your order ensure you provide the following information:

1. Your Antigen for affinity purifications only
2. Your Antibody containing solution
3. Your desired Purification
4. Our Offer, our order form or your official PO
5. VAT number (only EU Customers, not German Customers)