Peptide Antibodies

The major advantage of Peptide Antibodies is, that you can produce antibodies from proteins you only know the sequence from. There is no need for protein production or purification.

Peptide Prediction by Davids

With your amino acid sequence Davids researchers suggest you peptides, that represent your protein. Davids handles all your data confidentially. The complete service can be done by Davids, including peptide prediction, synthesis, conjugation, immunization and antibody purification. Alternatively you can synthesize your peptide in your lab and Davids performs the conjugation and/or the immunizations.

Conjugation of Peptides for better Immune Response

To improve the antigen presentation from small molecules, the peptides are conjugated covalently to a carrier like KLH, OVA, Thyroglobulin, BSA or other molecules. An affinity purification is recommended after the immunization to receive monospecific antibodies.

Peptide Predictions

Peptide Synthesis

Peptide Conjugation

Peptide Immunization

Order Process

Peptide Predictions

In combination with Davids Peptide Prediction Program, our scientists help you to find peptides that represent your protein (see info box). Take advantage of this free service by writing us an email with your amino acid sequence. All of your data is handled confidentially. We only start the synthesis of your peptide, when you confirm one or two of the suggested peptides for the antibody production.
Our prediction program in combination with our scientists suggest you peptides that represent your protein and that are suitable for the antibody production. These peptides have to be antigenic, soluble and must have good epitopes. For a higher chance to use the peptide that represents your protein in the best way, you can immunize one animal with two different peptides. The chance to use the best peptide increases from 70% (1 peptide) to 90% (2 peptides) to 97% (3 peptides).

Peptide Synthesis

The peptide production is done with solid phase synthesis. Quality controls are performed with HPLC and MS with each peptide. The purity is about 80% (70 –98%) for the immunizations. If you need a higher purity please ask for this service. The services listed in the table below are optimized for antibody production. Davids "All-Inclusive-Service" provides you with every step you need including the peptide synthesis and conjugation. If you need more or less amount of peptides please ask for this service.

	Stand Alone Synthesis	All Inclusive	Mixed Conjugation	High Purity
Cat.No	P104 (1 peptide)	P101 (1 peptide) P102 (2 peptides)	P111 (1 peptide)	P10X (1 peptide)
Schedule	2 - 5 weeks	2 - 5 weeks	2 - 5 weeks	2 - 5 weeks
Synthesis	up to 20 aa included	up to 20 aa included	up to 20 aa included	each aa
Maximal Length	up to 100 aa	up to 100 aa	up to 100 aa	up to 100 aa
Conjugation	No conjugation	1 Conjugation	3 Conjugations	No Conjugation
Carrier	-	KLH BSA Ovalbumin Thyroglobulin More	KLH BSA Ovalbumin Thyroglobulin More	-
Peptide
Purity	> 70%	> 70%	> 70%	> 95%
Amount	15 mg	15 mg	15 mg	15 mg
Quality Control
HPLC	Included	Included	Included	Included
MS	Included	Included	Included	Included
Application
Recommended for	For your applications Affinity Purification Depletion	Rabbit Antisera Mice, Rats, Guinea pig Antisera Chicken Egg Yolk Antibodies	Peptides with small antigenicity	Applications that require high purity
Price	Price List	Price List	Price List	On Demand
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Peptide Conjugation

You have the chance to send us your peptide for conjugation. Davids performs the conjugation you need. We offer the conjugation to KLH, Ovalbumin, BSA, Thyroglobulin or other carriers. The conjugation is performed at the -NH₂, -COOH, -SH, -OH or other groups of the amino acids, depending which amino acids are available at the peptide. To enhance the antigenicity multiple carriers or multiple conjugation methods can be used. This Mixed Conjugation (P111) includes the peptide synthesis and is recommended for peptides with low antigenicity. Davids All-Inclusive-Services P101 and P102 include the peptide synthesis, conjugation and quality control as well.

	Stand Alone Conjugation	All Inclusive	Mixed Conjugation
Cat.No	P105 (1 peptide)	P101 (1 peptide) P102 (2 peptides)	P111 (1 peptide)
Schedule	1 day	2 - 5 weeks	2 - 5 weeks
Synthesis	Peptide from Customer	up to 20 aa included	up to 20 aa included
Maximal Length	-	up to 100 aa	up to 100 aa
Conjugation	1 Conjugation	1 Conjugation	3 Conjugations
Carrier	KLH BSA Ovalbumin Thyroglobulin More	KLH BSA Ovalbumin Thyroglobulin More	KLH BSA Ovalbumin Thyroglobulin More
Peptide
Purity	-	> 70%	> 70%
Amount	-	15 mg	15 mg
Quality Control
HPLC	-	Included	Included
MS	-	Included	Included
Application
Recommended for	Rabbit Antisera Mice, Rats, Guinea pig Antisera Chicken Egg Yolk Antibodies	Rabbit Antisera Mice, Rats, Guinea pig Antisera Chicken Egg Yolk Antibodies	Peptides with small antigenicity
Price	Price List	Price List	Price List
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Peptide Immunization

Davids starts with the immunizations as soon as the peptide synthesis and preparation is finished. Please add one of our Custom Polyclonal Antibody Services for: Rabbits, Chicken, Small Rodents (Mice, Rats, Guinea pigs) or our Custom Monoclonal Antibody Services for Mice or Rats to your order.
We recommend an affinity purification for polyclonal antibodies to receive monospecific antibodies from the peptide immunization.

Additional Services

Additional services like the preparation of peptides are recommended, when the peptides are used for in vivo cell culture applications. Here the peptide fraction is diluted and the impurities are removed by HPLC. Vaporable substances are removed via lyophilization. In a third step the peptide fraction is diluted in a cell culture compatible buffer like PBS or water and sterile filtered.

Order Process

Davids keeps the order process very simple. For peptide antibodies you can send us your amio acid sequence (one letter amino acid code as txt, pdf or by email) and our scientists predict the antigenicity, solubility and epitopes. Davids only starts the synthesis when you confirm the peptide(s) by email, PO or FAX. If you want to send us peptides synthesized in your lab, send the peptide to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The immunizations usually start at the same day, when the antigen arrives. To fasten up the immunization and/or synthesis process and help us to connect your antigen/sequence with your order, ensure you provide the following information:

1. Your Peptide/Protein Sequence
2. Concentration & Buffer Conditions of your Peptide
3. Our Offer, our order form or your official PO
4. VAT number (only EU Customers, not German Customers)