Chicken Antibody Production

Egg Yolk and Chicken Serum

Chicken antibodies have a very high yield. Eggs are used to isolate all antibodies. Over time, a large number of antibodies can be generated since the amount of antibodies from 10 eggs is equivalent to the amount of antibodies from one rabbit. This reduces the amount of required antigen and is particularly animal friendly because a single chicken may create 42000 mg antibodies in a year. Thus, chicken antibodies are an excellent substitute for sheep and goat antibodies, especially when a significant quantity of antibodies is required.

Over the period of a year, one chicken's antibody production can replace up to 20 rabbits, 4 goats, or 4 sheep. Davids egg yolk antibodies are all ready to use. Davids PrepI preparation from egg yolk is equivalent to raw mammalian sera. Additional purifications are available to meet your specific requirements. All of the benefits of Chicken Egg Yolk Antibodies may be found here.

If you want to learn more about chicken egg yolk antibodies or if you want to take advantage of a special offer for a big quantity of antibodies, please send us an email: info@davids-bio.com or use our Antibody Creator to make your individual quote.

Antigen Requirements

The quantity of antigen required for chicken antibodies is very small in comparison to the amount of antibodies that can be obtained. The table below contains our antigen recommendations for the development of chicken egg yolk antibodies. However, antibody production can often be done with less antigen. In this situation, please request this service from us. Please contact us if you require any further information or if you have an antigen that does not appear in the table.

	Antigen	Amount	Instructions	Shipping
	Protein	0.5 - 1 mg 0.3 - 2 mg/ml	Customer provided proteins	-20°C (ice packs) to -80°C (dry-ice) or lyophilized
	Protein Expression	-	Expression Service by Davids	Ship Expression Plasmid at -20°C
	Protein (SDS-Gel)	0.5 - 1 mg 3 - 8 gel bands	Protein Extraction from SDS-gel pieces. Cut the protein specific gel bands from the gel.	Without addition of solutions at room temperature
	Peptides	1 - 2 mg 0.3 - 2 mg/ml	Customer provided peptides or Peptide synthesis and Conjugation Service by Davids.	Lyophilized or with ice packs
	Cells	106 - 107 cfu / immunization	Preparation with Davids Cell Immunization KIT for optimal presentation of the surface to the immune system.	Please contact us when you want to send cells
	DNA	1 - 2 mg 0.3 - 2 mg/ml	DNA preparation by Davids.	At room temperature or with ice packs
	Small Molecules	1 - 10 mg 0.3 - 2 mg/ml	Preparation of small molecules by Davids	Please contact us when you want to send small molecules
For Affinity Purification we need additional >0.5 mg protein, 3 mg peptide or 108 cfu

Immunizations

The immunization process begins as soon as your antigen arrives. Four immunizations are given before the eggs are collected and the antibodies are prepared. After receiving the initial wave of antibodies, the chicken is maintained alive for two weeks (up to day 77). During this period, you may determine if you need more antibodies or whether you wish to finish the project. When enough antigen is available, a fifth vaccination is performed to obtain the titer. Additional immunizations, egg harvests, and antibody preparations are all feasible over time.

Chickens will occasionally postpone egg laying for a short time period. In this instance, the antibody preparation is prolonged for a brief time. We reduce this probability by selecting healthy hens that are already laying eggs. The purification section contains an overview of our chicken egg yolk antibody services: Egg Yolk Purification.

Day	63 Day Protocol
1	Preimmune Egg Yolk IgG/IgY, approx. 90% pure
1	1. Immunization
14	2. Immunization
28	3. Immunization
35	4. Immunization
45-55	Collection of the eggs
56	Optional: 5. Immunization
63	Preparation of Eggs
77	Project Elongation or Termination
∞	Production of antibodies from up to 200 egg yolks per year.

Chicken Antibody Purification

All of our service packages feature ready-to-use chicken antibodies. PrepI antibodies are equivalent to raw mammalian serum and are provided in packages A001/A002. Purification using PrepII or ProteinL (included in A003) yields a pure total IgY fraction. This fraction may be preferable in a variety of situations.

Antigen specific affinity purifications are available for PrepI preparations. Simply add affinity purification to your purchase. We suggest low endotoxin, high specificity, and sterile filtered antibodies for usage in cell cultures (PrepIII). Send us an email at info@davids-bio.com if you have any questions or recommendations.

	PrepI	PrepII	PrepIII	Affinity Purification
Cat.No	A001 (incl. 1 hen) A002 (incl. 2 hens) A012	A003 (incl. 1 hen) R003	R006	R017
Duration	5 days	5 days	5 - 10 days	1 - 5 days
Product Information
Procedure	Preparation	Chromatography	Antigen bound to resin	Antigen bound to resin
Comparable with	Raw Serum	ProteinA	PrepIII	Affinity Purification
Product	Whole IgY fraction in buffer	High purity, whole IgY fraction	Specific Antibodies Low Endotoxin Sterile	Antigen Specific Antibodies
Purity	70 - 90%	90 - 98%
Amount (approx.)	10 eggs corresponds to 100 ml Serum	10 eggs corresponds to 100 ml Serum	0.1 - 10 mg (10 eggs)	0.5 - 10 mg (10 eggs)
Applications
Optimal for	ELISA	Immuno-Histo-Chemistry (IHC)	in vivo Cell Culture Applications Fluorescent Activated Cell Sorting (FACS)	WesternBlot Immuno-Histo-Chemistry (IHC) Fluorescent Activated Cell Sorting (FACS) ELISA
Price	Price List	Price List	Price List	Price List
Get your free offer now

Additional Services

Other immunization schedules and extra services are available for your antibody project. The chicken continues to produce eggs after the initial batch of egg yolk antibodies, and we may prepare additional antibody batches from the same animal on demand. Furthermore, the isolation of lymphocytes from spleen, bone marrow and other organs as well as the isolation of RNA and DNA is available.

Cat.No.	Description	Amount	Catalog Price
A012	Additional Immunization, Housing for 30 days, Collection and PrepI	10 eggs	Price List
R003	Additional Immunization, Housing for 30 days, Collection and PrepII	10 eggs	Price List
A705	ELISA Raw Data incl. graph	From 1 Preparation	Price List
	Final Bleed (Antiserum)	1 chicken	Price List
N101	Spleen Cell Isolation	1 chicken	Price List
N301	Bone Marrow Cell Isolation	1 chicken	Price List
T901	RNA Isolation from Cells (1 x 10^8)	1 organ	Price List
P075.010	anti-egg IgG matrix for immunoprecipitation	1 ml	Price List

Advantage of Egg Yolk Antibodies

The table compares the benefits of chicken egg yolk antibodies with mammalian serum antibodies. Considering IgG is a serum filtrate, it is identical to IgY. The antibodies can be utilized in the same way as mammalian sera.

	Advantage	Aspect	Solution
No binding at ProteinA/ProteinG	Less background	No ProteinA or ProteinG purification	Our special purification methods (PrepI, PrepII) turn to equal or better antibodies
No activation of complement factors	Less background	-
No binding to Fc of antibodies	Less background	-
No binding to rheumatoid factor	-	-
Lipids/Lipoproteins	-	Reduced activity	Purification like PrepI, PrepII or PrepIII to remove the lipids and lipoproteins
Yield of IgG	approx. 50 - 100 mg/egg results in 1 - 2 g per month	-

Order Process

The ordering procedure is straightforward. Send the antigen for immunization to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The immunizations usually start at the same day when the antigen arrives in our lab. Provide the following information to fasten up the start and help us to connect your antigen with your order

1. Your Antigen
2. Concentration & Buffer Conditions of your Antigen
3. Our Offer, our order form or your official PO
4. VAT number (only EU customers, not required for German customers)

You can generate your own quote with our Antibody Creator. When you prefer to order peptide antibodies, send us your protein sequence and we will recommend peptides that represent your protein. For further information, please visit: Peptide Antibodies