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Cells as antigen
Cells As Antigen
Antigen Overview

Whole Cells as Antigen

Using cells as antigen has huge advantages when you want to produce antibodies that detect whole cells or surface antigens. Cells can be used for the whole immunization process or as a last boost to show the immune system the native extracellular antigens. This will lead to more specific antibodies.
For the optimal presentation of the antigen from the cells we recommend our Cell Preparation KIT. You can do it in your lab or send us your cells and let us perform the cell preparation prior to immunizations.
Whole Cell

Cell Preparation for Immunization

Purification

Antibody Purification

Additional Information

Additional Information

Order Process

Order Process


Preparation of whole cells for immunization

For optimal presentation of the cell antigens use our Cell Immunization KIT. You can do the preparation in your lab or send us your inactivated cells and we perform it in our lab. The price is the same. We highly recommend that you perform the preparation in your lab with fresh cells. Though when you want us to do it please inactivate your cells i.e. with heat or UV-light. For additional information please send us an email: info@davids-bio.com. Table 1 shows the cell amount needed for the immunization of one animal.

Recommended cfu/pfu for the immunization of one animal.
Rabbit Chicken Mouse Rat Guinea Pig
Rabbit Antigen RecommendationsChicken Antigen RecommendationsMouse Antigen RecommendationsRat Antigen RecommendationsGuinea Pig Antigen Recommendations
Bacterial Cells (1 animal)5 x 106 cfu5 x 106 cfu5 x 106 cfu5 x 106 cfu5 x 106 cfu
Virus Particles (1 animal)107 pfu107 pfu107 pfu107 pfu107 pfu
Mammalian Cells (1 animal)107 - 108 cfu107 - 108 cfu107 - 108 cfu107 - 108 cfu107 - 108 cfu
Cell Preparation
Price List

Purification of Antibodies

Antibodies from whole cell antigens can be purified with ProteinA or with affinity purification. For the affinity purification we need additional 108 cfu/pfu to create the affinity matrix. For an overview of our purification methods go to our Purification Page.

Additional Information

Please keep in mind that ELISA titer determinations with whole cells may not show the real titer. For an optimal titer determination the cells have to bind to the ELISA plate. We have methods to bind cells to the ELISA plates though for each species or cell type different conditions are optimal. The real titer from whole cell antigens is usually higher than determined with our ELISA. You can send us the antigen protein only for ELSIA determinations if you need a more precise titer and the protein is available.

Order Process

We keep the order process very simple. Please use our Cell Immunization KIT or inactivate your cells i.e. with heat or UV-light efore you send them to us. Please contact us before you send infectious or pathological cells. You can send us your inactivated whole cells to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The Cell Immunization Preparation with our Cell Immunization KIT is done in our lab when we receive not prepared cells. The immunizations usually start within 2 - 3 days after your cells arrive in our lab. To fasten up the immunization process and help us to connect your antigen with your order ensure you provide the following information:

  1. Your Antigen
  2. Cell Amount
  3. Our Offer, our order form or your official PO
  4. VAT number (only EU Customers, not German Customers)

If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies

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Davids Biotechnologie GmbHemail:info@davids-bio.com
Roentgenstrasse 3phone:+49-941-948228
93055 Regensburgfax:+49-941-9468119
Germany