Polyclonal Antibodies

Polyclonal antibodies are the antibodies of choice when you need inexpensive and fast produced high affinity antibodies. Your polyclonal antibodies recognize multiple epitopes of your antigen, which can lead to a better detection in some applications. Multiple antibodies are able to bind your protein at different epitopes which leads to a signal amplification. This usually leads to better results in immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP).

Antigen Preparation

Immunization
 

Antibody Purification

Additional Services

Antigen

For the antibody production nearly any kind of antigen can be used. Usually proteins, peptides, SDS-Gel pieces, DNA, cells or small molecules are used for polyclonal or monoclonal antibodies.
When you have purified protein available, the native protein can be used as antigen. When the purification of the protein is difficult or when the purified protein shows multiple bands in a SDS-gel you can use our SDS-Gel Extraction service (Cat.No.A703). Here you cut your protein specific gel band from your gel and we extract the protein from it.
For proteins you only know the sequence from you can use our Peptide Antibody Service (P101). This service includes a peptide prediction to see, which peptides represent your protein in the best way. Our scientist with the help of our analytical program predict the antigenicity, solubility and epitopes of the peptides. We synthesize and conjugate the peptides to a carrier to improve the antigenicity.

If you have any questions regarding your antigens you can just send us an email: info@davids-bio.com

	Antigen	Cat.No.	Description	We need
	Protein	Provided by Customer	We use your protein for the immunization	Your antigen
	Protein Expression	E100	Protein Expression Service for antibody production	Your expression plasmid
	Protein (SDS-Gel)	A703	Protein Extraction from SDS-Gel pieces	Your protein in SDS-gel pieces
	Peptides	P101	Peptide Prediction, Synthesis & Conjugation	Your protein amino acid sequence
	Cells	K105	Preparation of cells for immunization	Your inactivated cells
	DNA	A702	Preparation of DNA for immunization	Your DNA
	Small Molecules	P105	Conjugation of the small molecules if needed	Your antigen

If you need further information or if you have another antigen that does not fit in the table please contact us.

Immunization

We start with the immunizations as soon as your antigen arrives or as soon as we finished the synthesis or preparation of your antigen. We offer the immunization of different species for your special needs. Most common are “New Zealand White Rabbits” for the production of your polyclonal antibodies. In addition we offer the immunization of chicken, mice, rats or guinea pigs. We are able to immunize one or multiple animals, depending how many antibodies are needed. Please ask for your special offer for a large number of animals.
We have different protocols for the needs of our customers. Our standard 63-day protocol fits to most applications. For customers who need their antibodies faster we offer our SuperFast procedure, which takes 28-days. Here we use a superior adjuvant and a proprietary immunization schedule.

	Animal	Product	Purification	Advantages
	Rabbit	40 - 90 ml antiserum	ProteinA, Affinity Purification
	Chicken	Egg Yolk from 8 eggs / 14 days	PrepI, PrepII, Affinity Purification	Large Amounts of Antibodies over time
	Mouse	0.2 - 1 ml antiserum	ProteinA, Affinity Purification	Monoclonal Antibody Production is possible
	Rat	2 - 10 ml antiserum	ProteinA, Affinity Purification	Monoclonal Antibody Production is possible
	Guinea pig	5 - 15 ml antiserum	ProteinA, Affinity Purification	Optimal choice for IHC
	Phosphospecific Antibodies	Peptide, Antiserum	Affinity Purification, Depletion	Recognition of phosphorylation site

Purification

We recommend purifications when you need specific antibodies especially for applications like ImmunoHistoChemistry (IHC), WesternBlot (WB), ImmunoPrecipitation (IP), Chromatin ImmunopreciPitation (ChIP) or Fluorescent Activated Cell Sorting (FACS). Even for other applications like ELISA purified antibodies have advantages. We offer different purification methods for your special needs:

	Cat.No.	Purification	Available for	Product	Advantages
	R007	Affinity Purification	Antisera, Egg Yolk	Specific Antibodies against your antigen	Higher concentration of your antigen specific antibodies, less background
	R008	ProteinA	Antisera	Whole IgG fraction	Specific and unspecific antibodies
	R101	Depletion	Antisera	Removes unwanted antibodies	Antisera without unwanted antibodies
	A012	PrepI	Egg Yolk	Removal of lipids, salts	Comparable with raw antisera from mammals
	R003	PrepII	Egg Yolk	Whole IgY fraction	Comparable with ProteinA purification
	R004	PrepIII	Antisera, Egg Yolk	Specific antibodies for cell culture use	Sterile & specific antibodies directly for your cell culture use

Additional Services

Davids offers a wide range of antibody processing like enzyme or dye conjugation, modification or fragmentation.

In addition we offer you to prepare cells from spleen, blood, lymph nodes or to prepare whole organs from immunized animals. We can ship the cells or organs to you or offer the preparation of RNA from the fresh isolated cells.